Structural characterisation of a perdeuteriomethylated exoploysaccharide by NMR spectroscopy: characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. Bulgaricus EU23

Harding, Lindsay P., Marshall, Valerie M., Elvin, M., Gu, Y. and Laws, Andrew P. (2003) Structural characterisation of a perdeuteriomethylated exoploysaccharide by NMR spectroscopy: characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. Bulgaricus EU23. Carbohydrate Research, 338 (1). pp. 61-67. ISSN 0008-6215

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Abstract

The exopolysaccharide (EPS) from Lactobacillus delbrueckii subsp. bulgaricus EU23 was perdeuteriomethylated and the perdeuteriomethylated EPS (pdm-EPS) purified by elution from a C18 Sep-Pak cartridge. Both 1D and 2D NMR spectra were recorded for the pdm-EPS and these were interpreted to provide assignments for the individual 1H and 13C resonances of the sugar residues of the repeating unit. Using a combination of the results from monomer analysis and linkage analysis of the native EPS and the ROESY and HMBC NMR spectra of the pdm-EPS the following structure has been determined for the repeating unit:

Information

URI:

https://eprints.hud.ac.uk/id/eprint/255

URI:

https://eprints.hud.ac.uk/id/eprint/255

URI:

https://eprints.hud.ac.uk/id/eprint/255

Title:

Structural characterisation of a perdeuteriomethylated exoploysaccharide by NMR spectroscopy: characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. Bulgaricus EU23

Creators:

Harding, Lindsay P., Marshall, Valerie M., Elvin, M., Gu, Y. and Laws, Andrew P.

Identification Number:

10.1016/S0008-6215(02)00354-3

Official URL:

http://dx.doi.org/10.1016/S0008-6215(02)00354-3

Date:

2003

Subjects:

Q Science > QD Chemistry

Schools:

School of Applied Sciences
School of Applied Sciences > Biomolecular Sciences Research Centre