Javid, Farideh A. and Palea, Stefano (2014) The effect of 5-HT and electrical field stimulation on the contractility of the whole isolated urinary bladder of Suncus murinus. European Journal of Pharmacology, 723. pp. 489-493. ISSN 0014-2999

The present study used the whole isolated urinary bladder of Suncus murinus, to investigate the effect of exogenously added serotonin (5-HT) and electrical field stimulation (EFS) in the absence and presence of methysergide, a 5-HT1/2/7 receptor antagonist or the selective 5-HT7 receptor antagonist, SB269970. Further experiments investigated the involvement of potassium channel, cholinergic and purinergic systems in mediating the contractile response to EFS. Pre-treatment with methysergide reduced and increased the contractile responses to 5-HT and EFS, respectively. Pre-treatment with SB269970 increased the responses to 5-HT without modifying the EFS-induced contractions. EFS-induced contractions were not modified by pre-treatment with atropine (10 μM), α-β-methylene ATP or glibenclamide. EFS-induced contractions were attenuated by cromakalim or atropine (<1 μM). In conclusion, the 5-HT2 receptors are likely to play a role in mediating the contractile response to 5-HT in detrusor muscle. Furthermore, EFS-induced contractions are mediated through cholinergic and an unknown neurotransmitter which is modulated by K(ATP ) channels in the detrusor muscle of Suncus murinus.

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