Brotherton, Paul, Endicott, Phillip, Beaumont, Mark, Barnett, Ross, Austin, Jeremy, Cooper, Alan and Sanchez, Juan J. (2008) Single primer extension (SPEX) amplification to accurately genotype highly damaged DNA templates. Forensic Science International: Genetics Supplement Series, 1 (1). pp. 19-21. ISSN 18751768

High levels of non-authentic sequence data can be generated by traditional PCR-based methodologies when DNA is damaged, template numbers are small and/or the target amplification size too large. We therefore present an alternate methodology based on single primer extension (SPEX) amplification; that places no pre-defined size constraints on amplification and interacts with only one of the DNA strands at the target locus.

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