Stockwin, Luke H., Matzow, Torkjel, Georgopoulos, Nikolaos T., Stanbridge, Lindsay J., Jones, Samantha V., Martin, Iain G., Blair-Zajdel, Maria E. and Blair, G. Eric (2002) Engineered expression of the Coxsackie B and adenovirus receptor (CAR) in human dendritic cells enhances recombinant adenovirus-mediated gene transfer. Journal of Immunological Methods, 259 (1-2). pp. 205-215. ISSN 0022-1759
Metadata only available from this repository.Abstract
Dendritic cells (DCs) are key antigen-presenting cells (APCs) that act as central modulators of cellular immune responses. Genetic modification of DCs has considerable therapeutic potential in the treatment of a wide spectrum of diseases, including cancer and persistent viral infection. In this report, we show that pre-treatment of DCs with a recombinant adenovirus encoding the major adenovirus receptor, Coxsackie B and adenovirus receptor (CAR), significantly increased the uptake of recombinant adenoviruses (Ads) by primary immature monocyte-derived DCs. This could be correlated with CAR mRNA and surface protein expression. Transduction of DCs by recombinant adenoviruses did not significantly alter cellular viability. Therefore, we propose that pre-treatment of DCs with Ad5-CAR is one strategy to increase the susceptibility of DCs to transduction by recombinant Ads.
Item Type: | Article |
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Subjects: | Q Science > QH Natural history > QH301 Biology |
Schools: | School of Applied Sciences |
Related URLs: | |
Depositing User: | Cherry Edmunds |
Date Deposited: | 01 Dec 2009 16:51 |
Last Modified: | 28 Aug 2021 10:53 |
URI: | http://eprints.hud.ac.uk/id/eprint/6475 |
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