Thermodynamics of Binding of 2-Methoxy-3-isopropylpyrazine and 2-Methoxy-3-isobutylpyrazine to the Major Urinary Protein

Bingham, Richard, Findlay, John B. C., Hsieh, Shih-Yang, Kalverda, Arnout P., Kjellberg, Alexandra, Perazzolo, Chiara, Phillips, Simon E. V., Seshadri, Kothandaraman, Trinh, Chi H., Turnbull, W. Bruce, Bodenhausen, Geoffrey and Homans, Steve W. (2004) Thermodynamics of Binding of 2-Methoxy-3-isopropylpyrazine and 2-Methoxy-3-isobutylpyrazine to the Major Urinary Protein. Journal of the American Chemical Society, 126 (6). pp. 1675-1681. ISSN 1520-5126

Metadata only available from this repository.

Abstract

In the present study we examine the thermodynamics of binding of two related pyrazine-derived ligands to the major urinary protein, MUP-I, using a combination of isothermal titration calorimetry (ITC), X-ray crystallography, and NMR backbone 15N and methyl side-chain 2H relaxation measurements. Global thermodynamics data derived from ITC indicate that binding is driven by favorable enthalpic contributions, rather than the classical entropy-driven hydrophobic effect. Unfavorable entropic contributions from the protein backbone and side-chain residues in the vicinity of the binding pocket are partially offset by favorable entropic contributions at adjacent positions, suggesting a “conformational relay” mechanism whereby increased rigidity of residues on ligand binding are accompanied by increased conformational freedom of side chains in adjacent positions. The principal driving force governing ligand affinity and specificity can be attributed to solvent-driven enthalpic effects from desolvation of the protein binding pocket.

Item Type:	Article
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology
Schools:	School of Applied Sciences
Related URLs:	Author
Depositing User:	Graham Stone
Date Deposited:	09 Oct 2009 09:11
Last Modified:	09 Oct 2009 09:11
URI:	http://eprints.hud.ac.uk/id/eprint/5830

Downloads

Repository Staff Only: item control page

View Item