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Quantification of three macrolide antibiotics in pharmaceutical lots by HPLC: Development, validation and application to a simultaneous separation

Mahmoudi, A, De Francia, Silvia, Boukhechem, M and Pirro, Elisa (2016) Quantification of three macrolide antibiotics in pharmaceutical lots by HPLC: Development, validation and application to a simultaneous separation. British Journal of Pharmacy, 1 (1). ISSN 2058-8356

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Abstract

A new validated high performance liquid chromatographic (HPLC) method with rapid analysis time and high efficiency, for the analysis of erythromycin, azithromycin and spiramycin, under isocratic conditions with ODB RP18 as a stationary phase is described. Using an eluent composed of acetonitrile –2-methyl-2-propanol –hydrogenphosphate buffer, pH 6.5, with 1.5% triethylamine (33:7: up to 100, v/v/v), delivered at a flow-rate of 1.0 mL min-1. Ultra Violet (UV) detection is performed at 210 nm. The selectivity is satisfactory enough and no problematic interfering peaks are observed. The procedure is quantitatively characterized and repeatability, linearity, detection and quantification limits are very satisfactory. The method is applied successfully for the assay of the studied drugs in pharmaceutical dosage forms as tablets and powder for oral suspension. Recovery experiments revealed recovery of 97.13–100.28%.

Item Type: Article
Subjects: R Medicine > RS Pharmacy and materia medica
Schools: School of Applied Sciences
Depositing User: Megan Taylor
Date Deposited: 15 Nov 2016 09:28
Last Modified: 17 Nov 2016 00:58
URI: http://eprints.hud.ac.uk/id/eprint/30146

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