Increased cutaneous absorption reflects impaired barrier function of reconstructed skin models mimicking keratinisation disorders

Eckl, K.M., Weindl, G., Ackermann, K., Küchler, S., Casper, R., Radowski, M.R., Haag, R., Hennies, Hans C. and Schäfer-Korting, M. (2014) Increased cutaneous absorption reflects impaired barrier function of reconstructed skin models mimicking keratinisation disorders. Experimental dermatology, 23 (4). pp. 286-288. ISSN 1600-0625

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Abstract

The aim of this study was to assess a recently established 3D model of congenital ichthyosis, representing severe epidermal barrier function defects, for skin penetration and permeation. We have generated disease models by knock-down of either TGM1 or ALOXE3 in primary human keratinocytes, and using keratinocytes and fibroblasts from patients with congenital ichthyosis. The results indicate disturbed barrier function as demonstrated by increased permeation of testosterone and caffeine particularly in TGM1 knock-down models compared to control models. In addition, enhanced penetration of the model dye nile red incorporated into solid lipid nanoparticles and core-multishell nanotransporters, respectively, was evident in disease models. Thus, in vitro skin disease models reproduce differences in barrier permeability and function seen in congenital ichthyosis and pave the way to personalised disease models. Furthermore, our findings indicate that nanocarriers may be useful in new, topical therapeutic approaches for the currently very limited treatment of congenital ichthyosis.

Item Type:	Article
Additional Information:	Unmapped bibliographic data: ST - Increased cutaneous absorption reflects impaired barrier function of reconstructed skin models mimicking keratinisation disorders [Field not mapped to EPrints] AD - Dermatogenetics, Division of Human Genetics and Department of Dermatology and Venereology, Innsbruck Medical University, Innsbruck, Austria; Dermatogenetics, Cologne Center for Genomics, University of Cologne, Cologne, Germany. [Field not mapped to EPrints] AN - 24612062 [Field not mapped to EPrints]
Uncontrolled Keywords:	3T3 Cells, nanoparticles
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics
Schools:	School of Applied Sciences
Depositing User:	Hans Hennies
Date Deposited:	08 Jul 2016 13:48
Last Modified:	29 Jul 2016 13:03
URI:	http://eprints.hud.ac.uk/id/eprint/28763

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