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Detecting errors in mtDNA data by phylogenetic analysis

Bandelt, Hans-Jurgen, Lahermo, P., Richards, Martin and Macaulay, Vincent (2001) Detecting errors in mtDNA data by phylogenetic analysis. International Journal of Legal Medicine, 115 (2). pp. 64-69. ISSN 0937-9827

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Sequencing and documenting a sample of homologous DNA stretches is prone to copying errors in a way rather analogous to the biological replication process. Previous attempts at obtaining representative mtDNA sequences, typically of the control region, for evolutionary studies or forensic purposes have yielded rather unsatisfactory results in many cases. The key ingredient in pinpointing problems with given data is the phylogenetic analysis of closely related mtDNAs within the framework of an established worldwide phylogeny that is supported by coding region information. We develop some general rules by which likely errors in data tables can readily be detected without rereading whole sequences repeatedly. Following these guidelines, one can expect to lower the error rate by at least an order of magnitude, although it will still be hard to beat the mitochondrial gamma polymerase in precision.

Item Type: Article
Subjects: Q Science > QP Physiology
Schools: School of Applied Sciences
Depositing User: Catherine Parker
Date Deposited: 11 Nov 2008 12:43
Last Modified: 28 Aug 2021 10:42


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