Harding, S. E., Abdelhameed, A. S., Gillis, R. B., Morris, Gordon and Adams, G. G. (2015) Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods. Methods in Molecular Biology, 1331. pp. 211-227. ISSN 1064-3745

Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), and capillary viscometry—intrinsic viscosity measurement. The chapter highlights the general principle of these five methods, describes experimental details, and specifies advances in the last years.

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