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Macromolecular conformation of chitosan in dilute solution: A new global hydrodynamic approach

Morris, Gordon, Castile, J., Smith, A., Adams, G. G. and Harding, S. E. (2009) Macromolecular conformation of chitosan in dilute solution: A new global hydrodynamic approach. Carbohydrate Polymers, 76 (4). pp. 616-621. ISSN 0144-8617

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Chitosans of different molar masses were prepared by storing freshly prepared samples for up to 6 months at either 4, 25 or 40 °C. The weight-average molar masses, Mw and intrinsic viscosities, [η] were then measured using size exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALLS) and a "rolling ball" viscometer, respectively. The solution conformation of chitosan was then estimated from:(a)the Mark-Houwink-Kuhn-Sakurada (MHKS) power law relationship [η] = kMwa and(b)the persistence length, Lp calculated from a new approach based on equivalent radii [Ortega, A., & Garcia de la Torre, J. (2007). Equivalent radii and ratios of radii from solution properties as indicators of macromolecular conformation, shape, and flexibility. Biomacromolecules, 8, 2464-2475]. Both the MHKS power law exponent (a = 0.95 ± 0.01) and the persistence length (Lp = 16 ± 2 nm) are consistent with a semi-flexible rod type (or stiff coil) conformation for all 33 chitosans studied. A semi-flexible rod conformation was further supported by the Wales-van Holde ratio, the translational frictional ratio and sedimentation conformation zoning. © 2008 Elsevier Ltd. All rights reserved.

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Item Type: Article
Uncontrolled Keywords: Chitosan Equivalent radii Intrinsic viscosity Molar mass Molar weight Sedimentation coefficient Semi-flexible rod conformation Biological membranes Chitin Chromatographic analysis Fluid dynamics Hydrogels Land use Light scattering Macromolecules Multilayers Polyethylene terephthalates Size exclusion chromatography Viscosity
Subjects: Q Science > QD Chemistry
Schools: School of Applied Sciences
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References: Language of Original Document: English Correspondence Address: Morris, G.A.; National Centre for Macromolecular Hydrodynamics, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, LE12 5RD, United Kingdom; email: References: Berth, G., Dautzenberg, H., Peter, M.G., Physico-chemical characterization of chitosans varying in degree of acetylation (1998) Carbohydrate Polymers, 36, pp. 205-216; Bohdanecky, M., New method for estimating the parameters of the wormlike chain model from the intrinsic viscosity of stiff-chain polymers (1983) Macromolecules, 16, pp. 1483-1493; Brugnerotto, J., Desbrières, J., Roberts, G., Rinaudo, M., Characterization of chitosan by steric exclusion chromatography (2001) Polymer, 42, pp. 9921-9927; Bushin, S.V., Tsvetkov, V.N., Lysenko, Y.B., Emel'yanov, V.N., Conformational properties and rigidity of molecules of ladder polyphenylsiloxane in solutions according the data of sedimentation-diffusion analysis and viscometry (1981) Vysokomolekulyarnye Soedineniya, A23, pp. 2494-2503; Cölfen, H., Berth, G., Dautzenberg, H., Hydrodynamic studies on chitosans in aqueous solution (2001) Carbohydrate Polymers, 45, pp. 373-383; Errington, N., Harding, S.E., Vårum, K.M., Illum, L., Hydrodynamic characterisation of chitosans varying in degree of acetylation (1993) International Journal of Biological Macromolecules, 15, pp. 113-117; Fee, M., Errington, N., Jumel, K., Illum, L., Smith, A., Harding, S.E., Correlation of SEC/MALLS with ultracentrifuge and viscometric data for chitosans (2003) European Biophysical Journal, 32, pp. 457-464; Gralén, N., (1944) Sedimentation and diffusion measurements on cellulose and cellulose derivatives, , PhD Dissertation, University of Uppsala, Sweden; Harding, S.E., The Intrinsic viscosity of biological macromolecules. Progress in measurement, interpretation and application to structure in dilute solution (1997) Progress in Biophysics and Molecular Biology, 68, pp. 207-262; Harding, S.E., Analysis of polysaccharides size, shape and interactions (2005) Analytical ultracentrifugation techniques and methods, pp. 231-252. , Scott D.J., Harding S.E., and Rowe A.J. (Eds), Royal Society of Chemistry, Cambridge; Harding, S.E., Berth, G., Ball, A., Mitchell, J.R., Garcìa de la Torre, J., The molar mass distribution and conformation of citrus pectins in solution studied by hydrodynamics (1991) Carbohydrate Polymers, 168, pp. 1-15; Harding, S.E., Davis, S.S., Deacon, M.P., Fiebrig, I., Biopolymer mucoadhensives (1999) Biotechnology and genetic engineering reviews, 16, pp. 41-86. , Harding S.E. (Ed), Intercept: Andover, UK; Harding, S.E., Vårum, K.M., Stokke, B.T., Smidsrød, O., Molar mass determination of polysaccharides (1991) Advances in carbohydrate analysis, 1, pp. 63-144. , White C.A. (Ed), JAI Press Limited: Greenwich, USA; Kasaai, M.R., Calculation of Mark-Houwink-Sakurada (MHS) equation viscometric constants for chitosan in any solvent-temperature system using experimental reported viscometric constants data (2006) Carbohydrate Polymers, 68, pp. 477-488; Kratky, O., Porod, G., Röntgenungtersuchung gelöster fadenmoleküle (1949) Recueil Des Travaux Chimiques Des Pays-Bas, 68, pp. 1106-1109; Lamarque, G., Lucas, J.-M., Viton, C., Domard, A., Physicochemical behavior of homogeneous series of acetylated chitosans in aqueous solution: Role of various structural parameters (2005) Biomacromolecules, 6, pp. 131-142; Mazeau, K., Rinaudo, M., The prediction of the characteristics of some polysaccharides from molecular modelling. Comparison with effective behaviour (2004) Food Hydrocolloids, 18, pp. 885-898; Morris, G.A., Foster, T.J., Harding, S.E., The effect of degree of esterification on the hydrodynamic properties of citrus pectin (2000) Food Hydrocolloids, 14, pp. 227-235; Morris, G.A., García de al Torre, J., Ortega, A., Castile, J., Smith, A., Harding, S.E., Molecular flexibility of citrus pectins by combined sedimentation and viscosity analysis (2008) Food Hydrocolloids, 22, pp. 1435-1442; Ortega, A., García de la Torre, J., Equivalent radii and ratios of radii from solution properties as indicators of macromolecular conformation, shape, and flexibility (2007) Biomacromolecules, 8, pp. 2464-2475; Pavlov, G.M., Harding, S.E., Rowe, A.J., Normalized scaling relations as a natural classification of linear macromolecules according to size (1999) Progress in Colloid and Polymer Science, 113, pp. 76-80; Pavlov, G.M., Rowe, A.J., Harding, S.E., Conformation zoning of large molecules using the analytical ultracentrifuge (1997) Trends in Analytical Chemistry, 16, pp. 401-405; Ralston, G., (1993) Introduction to analytical ultracentrifugation, , Beckman Instruments Inc, Palo Alto [pp. 27-28]; Rinaudo, M., Chitin and chitosan: Properties and applications (2006) Progress in Polymer Science, 31, pp. 603-632; Rinaudo, M., Milas, M., Le Dung, P., Characterization of chitosan. Influence of ionic strength and degree of acetylation on chain expansion (1993) International Journal of Biological Macromolecules, 15, pp. 281-285; Rowe, A.J., The concentration dependence of transport processes: a general description applicable to the sedimentation, translational diffusion and viscosity coefficients of macromolecular solutes (1977) Biopolymers, 16, pp. 2595-2611; Schatz, S., Viton, C., Delair, T., Pichot, C., Domard, A., Typical physicochemical behaviors of chitosan in aqueous solution (2003) Biomacromolecules, 4, pp. 641-648; Schuck, P., Sedimentation analysis of noninteracting and self-associating solutes using numerical solutions to the Lamm equation (1998) Biophysical Journal, 75, pp. 1503-1512; Schuck, P., Diffusion-deconvoluted sedimentation coefficient distributions for the analysis of interacting and non-interacting protein mixtures (2005) Analytical ultracentrifugation techniques and methods, pp. 26-50. , Scott D.J., Harding S.E., and Rowe A.J. (Eds), Royal Society of Chemistry, Cambridge; Solomon, O.F., Ciutâ, I.Z., Détermination de la viscosité intrinsèque de solutions de polymères par une simple détermination de la viscosité (1962) Journal of Applied Polymer Science, 24, pp. 683-686; Tanford, C., (1961) Physical chemistry of macromolecules, , John Wiley and Sons, New York; Terbojevich, M., Cosani, A., Conio, G., Marsano, E., Bianchi, E., Chitosan: Chain rigidity and mesophase formation (1991) Carbohydrate Research, 209, pp. 251-260; Tombs, M.P., Harding, S.E., (1998) Polysaccharide biotechnology, , Taylor Francis, London, UK pp. 144-151; Velásquez, C.L., Albornoz, J.S., Barrios, E.M., Viscosimetric studies of chitosan nitrate and chitosan chlorhydrate in acid free NaCl aqueous solution (2008) E-Polymers, p. 014. ,, Available from; Vold, I.M.N., (2004) Periodate oxidised chitosans: Structure and solution properties, , PhD Dissertation, Norwegian University of Science and Technology, Trondheim, Norway; Wales, M., van Holde, K.E., The concentration dependence of the sedimentation constants of flexible macromolecules (1954) Journal of Polymer Science, 14, pp. 81-86; Yamakawa, H., Fujii, M., Translational friction coefficient of wormlike chains (1973) Macromolecules, 6, pp. 407-415
Depositing User: Gordon Morris
Date Deposited: 11 Dec 2012 12:21
Last Modified: 28 Aug 2021 20:17

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