Computing and Library Services - delivering an inspiring information environment

The effect of prolonged storage at different temperatures on the particle size distribution of tripolyphosphate (TPP)-chitosan nanoparticles

Morris, Gordon, Castile, Jonathan, Smith, Alan, Adams, Gary and Harding, Stephen E. (2011) The effect of prolonged storage at different temperatures on the particle size distribution of tripolyphosphate (TPP)-chitosan nanoparticles. Carbohydrate Polymers, 84 (4). pp. 1430-1434. ISSN 0144-8617

This is the latest version of this item.

[img] PDF - Accepted Version
Download (1MB)


Chitosan nanoparticles prepared by ionotropic gelation with the tripolyphosphate (TPP) polyanion have been widely considered for drug delivery. The stability (shelf-life) of TPP-chitosan nanoparticles is highly relevant to its potential use as a drug delivery agent as this plays an important role in the function of the nanoparticle and will determine shelf-life. In the present study, the physical stability (in terms of particle size) of TPP-chitosan nanoparticles was measured across a range of different temperature conditions: 4 °C, 25 °C and 40 °C using differential sedimentation. After 12 months storage at 4 and 25 °C the size of nanoparticles remained similar to those of the freshly prepared samples, whilst after storage at 40 °C there were little or no TPP-chitosan nanoparticles remaining after only 6 months. This may be due to the decrease in molar mass of the chitosan possibly due to hydrolysis causing scission of the polymer chains, which results in a decrease in nanoparticle size and eventual disintegration. This mechanism is important in the application of TPP-chitosan as a drug delivery agent. © 2011 Elsevier Ltd. All rights reserved.

▼ Jump to Download Statistics
Item Type: Article
Additional Information: NOTICE: this is the author’s version of a work that was accepted for publication in Carbohydrate Polymers. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Carbohydrate Polymers Volume 84, Issue 4, 2 April 2011, Pages 1430–1434
Uncontrolled Keywords: Chitosan nanoparticles Degradation Particle size Stability Tripolyphosphate (TPP) Ionotropic gelation Nanoparticle sizes Physical stability Polyanions Polymer chains Shelf life Temperature conditions Tripolyphosphates Chitin Chitosan Coagulation Drug delivery Gelation Particle size analysis Nanoparticles
Subjects: Q Science > QD Chemistry
Schools: School of Applied Sciences
Related URLs:
References: Abdelwahed, W., Degobert, G., Stainmesse, S., Fessi, H., Freeze-drying of nanoparticles: Formulation, process and storage considerations (2006) Advanced Drug Delivery Reviews, 58 (15), pp. 1688-1713. , DOI 10.1016/j.addr.2006.09.017, PII S0169409X06001840; Anitha, A., Deepa, N., Chennazhi, K.P., Nair, S.V., Tamura, H., Jayakumar, R., Development of mucoadhesive thiolated chitosan nanoparticles for biomedical applications (2011) Carbohydrate Polymers, 83, pp. 66-73; Berkland, C., King, M., Cox, A., Kim, K., Pack, D.W., Precise control of PLG microsphere size provides enhanced control of drug release rate (2002) Journal of Controlled Release, 82 (1), pp. 137-147. , DOI 10.1016/S0168-3659(02)00136-0, PII S0168365902001360; Davis, S.S., Illum, L., Chitosan for oral delivery of drugs (2000) Chitosan per Os: From Dietary Supplement to Drug Carrier, pp. 137-164; Dyer, A.M., Hinchcliffe, M., Watts, P., Castile, J., Jabbal-Gill, I., Nankervis, R., Smith, A., Illum, L., Nasal delivery of insulin using novel chitosan based formulations: A comparative study in two animal models between simple chitosan formulations and chitosan nanoparticles (2002) Pharmaceutical Research, 19 (7), pp. 998-1008. , DOI 10.1023/A:1016418523014; Eriksson, J.H.C., Hinrichs, W.L.J., De Jong, G.J., Somsen, G.W., Frijlink, H.W., Investigations into the stabilization of drugs by sugar glasses: III. The influence of various high-pH buffers (2003) Pharmaceutical Research, 20 (9), pp. 1437-1443. , DOI 10.1023/A:1025762328267; Fernandez-Urrusuno, R., Calvo, P., Remunan-Lopez, C., Vila-Jato, J.L., Alonso, M.J., Enhancement of nasal absorption of insulin using chitosan nanoparticles (1999) Pharmaceutical Research, 16 (10), pp. 1576-1581. , DOI 10.1023/A:1018908705446; Gan, Q., Wang, T., Chitosan nanoparticle as protein delivery carrier-Systematic examination of fabrication conditions for efficient loading and release (2007) Colloids and Surfaces B: Biointerfaces, 59 (1), pp. 24-34. , DOI 10.1016/j.colsurfb.2007.04.009, PII S0927776507001671; Gan, Q., Wang, T., Cochrane, C., McCarron, P., Modulation of surface charge, particle size and morphological properties of chitosan-TPP nanoparticles intended for gene delivery (2005) Colloids and Surfaces B: Biointerfaces, 44 (2-3), pp. 65-73. , DOI 10.1016/j.colsurfb.2005.06.001, PII S0927776505001682; Harding, S.E., Davis, S.S., Deacon, M.P., Fiebrig, I., Biopolymer mucoadhensives (1999) Biotechnology and Genetic Engineering Reviews, 16, pp. 41-86; Hu, B., Pan, C., Sun, Y., Hou, Z., Ye, H., Hu, B., Optimization of fabrication parameters to produce chitosan- tripolyphosphate nanoparticles for delivery of tea catechins (2008) Journal of Agricultural and Food Chemistry, 56, pp. 7451-7458; Illum, L., Chitosan and its use as a pharmaceutical excipient (1998) Pharmaceutical Research, 15 (9), pp. 1326-1331. , DOI 10.1023/A:1011929016601; Janes, K.A., Calvo, P., Alonso, M.J., Polysaccharide colloidal particles as delivery systems for macromolecules (2001) Advanced Drug Delivery Reviews, 47 (1), pp. 83-97. , DOI 10.1016/S0169-409X(00)00123-X, PII S0169409X0000123X; Jang, K.I., Lee, H.G., Stability of chitosan nanoparticles for l-ascorbic acid during heat treatment in aqueous solution (2008) Journal of Agricultural and Food Chemistry, 56, pp. 1936-1941; Laidlaw, I., Steinmetz, M., Introduction to differential sedimentation (2005) Analytical Ultracentrifugation Techniques and Methods, pp. 270-290; Lopez-Leon, T., Carvalho, E.L.S., Seijo, B., Ortega-Vinuesa, J.L., Bastos-Gonzalez, D., Physicochemical characterization of chitosan nanoparticles: Electrokinetic and stability behavior (2005) Journal of Colloid and Interface Science, 283 (2), pp. 344-351. , DOI 10.1016/j.jcis.2004.08.186, PII S0021979704009622; Luangtana-anan, M., Opanasopit, P., Ngawhirunpat, T., Nunthanid, J., Sriamornsak, P., Limmatvapirat, S., Lim, L.Y., Effect of chitosan salts and molecular weight on a nanoparticulate carrier for therapeutic protein (2005) Pharmaceutical Development and Technology, 10 (2), pp. 189-196. , DOI 10.1081/PDT-200054388; Lundqvist, M., Stigler, J., Elia, G., Lynch, I., Cedervall, T., Dawson, K.A., Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts (2008) Proceedings of the National Academy of Sciences of the United States of America, 105, pp. 14265-14270; Morris, G.A., Castile, J., Smith, A., Adams, G.G., Harding, S.E., The kinetics of chitosan depolymerisation at different temperatures (2009) Polymer Degradation and Stability, 94, pp. 1344-1348; Morris, G.A., Kök, M.S., Harding, S.E., Adams, G.G., Polysaccharide drug delivery systems based on pectin and chitosan (2010) Biotechnology and Genetic Engineering Reviews, 27, pp. 257-284; Muzzarelli, R.A.A., Genipin-crosslinked chitosan hydrogels as biomedical and pharmaceutical aids (2009) Carbohydrate Polymers, 77, pp. 1-9; Nasti, A., Zaki, N.M., De Leonardis, P., Ungphaiboon, S., Sansongsak, P., Rimoli, M.G., Chitosan/TPP and chitosan/TPP-hyaluronic acid nanoparticles: Systematic optimisation of the preparative process and preliminary biological evaluation (2009) Pharmaceutical Research, 26, pp. 1918-1930; Rinaudo, M., Chitin and chitosan: Properties and application (2006) Progress in Polymer Science, 31, pp. 603-632; Rinaudo, M., Milas, M., Le Dung, P., Characterization of chitosan. Influence of ionic strength and degree of acetylation on chain expansion (1993) International Journal of Biological Macromolecules, 15, pp. 281-285; Shu, X.Z., Zhu, K.J., A novel approach to prepare tripolyphosphate/chitosan complex beads for controlled release drug delivery (2000) International Journal of Pharmaceutics, 201 (1), pp. 51-58. , DOI 10.1016/S0378-5173(00)00403-8, PII S0378517300004038; Stokes, G.G., (1880) Mathematical and Physical Papers, 1. , Cambridge University Press Cambridge; Tang, E.S.K., Huang, M., Lim, L.Y., Ultrasonication of chitosan and chitosan nanoparticles (2003) International Journal of Pharmaceutics, 265 (1-2), pp. 103-114. , DOI 10.1016/S0378-5173(03)00408-3; Tsai, M.L., Bai, S.W., Chen, R.H., Cavitation effects versus stretch effects resulted in different size and polydispersity of ionotropic gelation chitosan-sodium tripolyphosphate nanoparticle (2008) Carbohydrate Polymers, 71 (3), pp. 448-457. , DOI 10.1016/j.carbpol.2007.06.015, PII S0144861707003335; Tsai, M.L., Chen, R.H., Bai, S.W., Chen, W.Y., The storage stability of chitosan/tripolyphosphate nanoparticles in a phosphate buffer (2011) Carbohydrate Polymers, 84, pp. 756-761; Vrum, K.M., Anthonsen, M.W., Grasdalen, H., Smidsrød, O., Determination of the degree of N-acetylation and distribution of N-acetyl groups in partially N-deacteylated chitins (chitosans) by high-field NMR spectroscopy (1991) Carbohydrate Research, 211, pp. 17-23; Vrum, K.M., Anthonsen, M.W., Grasdalen, H., Smidsrød, O., 13C NMR studies of the acetylation sequences in partially N-deacteylated chitins (chitosans) (1991) Carbohydrate Research, 217, pp. 19-27; Xu, Y., Du, Y., Effect of molecular structure of chitosan on protein delivery properties of chitosan nanoparticles (2005) International Journal of Pharmaceutics, 250, pp. 215-226; Zhang, H., Oh, M., Allen, C., Kumacheva, E., Monodisperse chitosan nanoparticles for mucosal drug delivery (2004) Biomacromolecules, 5 (6), pp. 2461-2468. , DOI 10.1021/bm0496211
Depositing User: Gordon Morris
Date Deposited: 13 Sep 2012 14:38
Last Modified: 28 Aug 2021 20:32

Available Versions of this Item

  • The effect of prolonged storage at different temperatures on the particle size distribution of tripolyphosphate (TPP)-chitosan nanoparticles. (deposited 13 Sep 2012 14:38) [Currently Displayed]


Downloads per month over past year

Repository Staff Only: item control page

View Item View Item

University of Huddersfield, Queensgate, Huddersfield, HD1 3DH Copyright and Disclaimer All rights reserved ©