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Reliable measurements of the size distributions of starch molecules in solution: Current dilemmas and recommendations

Gidley, M. J., Hanashiro, I., Hani, N. M., Hill, S. E., Huber, A., Jane, J. L., Liu, Q., Morris, Gordon, Rolland-Sabate, A., Striegel, A. M. and Gilbert, R. G. (2010) Reliable measurements of the size distributions of starch molecules in solution: Current dilemmas and recommendations. Carbohydrate Polymers, 79 (2). pp. 255-261. ISSN 0144-8617

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Characterisation of the distribution functions describing size-related parameters of individual whole starch molecules in solution is important for establishing biosynthesis-processing-structure-property relations, for improvements in human and animal nutrition, and for industrial applications of starch. Current techniques for obtaining these distributions suffer from a number of problems in dissolution procedures, size separation, detection, data processing and data reporting. The need is for techniques that have been shown to give an unbiased sampling of the distribution of the starch in its original state, and which are reproducible (i.e., the same results can be obtained with a given sample in a different laboratory and different experimental set-up). The problems with current methodologies are summarized here by an IUPAC-sponsored international group of specialists in the field. Steps to resolving these problems are also discussed. © 2009 Elsevier Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Analytical ultracentrifuge Field-flow fractionation Molecular weight Multiple-angle laser light scattering Size-exclusion chromatography Starch Characterisation Current techniques Data reporting Experimental setup International group Reliable measurement Starch molecules Structure property relation Biochemistry Centrifugation Centrifuges Chromatographic analysis Data processing Dissolution Distribution functions Industrial applications Light scattering Liquid chromatography Refraction Size separation Size exclusion chromatography Animalia
Subjects: Q Science > QD Chemistry
Schools: School of Applied Sciences
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References: Cited By (since 1996): 14 Export Date: 12 May 2011 Source: Scopus CODEN: CAPOD doi: 10.1016/j.carbpol.2009.07.056 Language of Original Document: English Correspondence Address: Gilbert, R.G.; Centre for Nutrition and Food Sciences, Hartley Teakle Bld., University of Queensland, Brisbane, QLD 4072, Australia; email: References: Aberle, T., Burchard, W., Vorwerg, W., Radosta, S., Conformational contributions of amylose and amylopectin to the structural-properties of starches from various sources (1994) Starch-Stärke, 46, pp. 329-335; Bello-Perez, L.A., Colonna, P., Roger, P., Paredes-Lopez, O., Macromolecular features of amaranth starch (1998) Cereal Chemistry, 75, pp. 395-402; Bello-Perez, L.A., Roger, P., Colonna, P., Paredes-Lopez, O., Laser light scattering of high amylose and high amylopectin materials, stability in water after microwave dispersion (1998) Carbohydrate Polymers, 37, pp. 383-394; Brewer, A.K., Striegel, A.M., Particle size characterization by quadruple-detector hydrodynamic chromatography (2009) Analytical and Bioanalytical Chemistry, 393, pp. 295-302; Brown, P.H., Schuck, P., Macromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugation (2006) Biophysical Journal, 90, pp. 4651-4661; Buleon, A., Colonna, P., Planchot, V., Ball, S., Starch granules: Structure and biosynthesis (1998) International Journal of Biological Macromolecules, 23, pp. 85-112; Bultosa, G., Hamaker, B.R., BeMiller, J.N., An SEC-MALLS study of molecular features of water-soluble amylopectin and amylose of Tef [Eragrostis tef (Zucc.) 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Depositing User: Gordon Morris
Date Deposited: 19 May 2011 11:03
Last Modified: 28 Aug 2021 11:06


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