Computing and Library Services - delivering an inspiring information environment

Pectic polysaccharides from Biophytum petersianum Klotzsch, and their activation of macrophages and dendritic cells

Inngjerdingen, M., Inngjerdingen, K. T., Patel, T. R., Allen, S., Chen, X., Rolstad, B., Morris, Gordon, Harding, S. E., Michaelsen, T. E., Diallo, D. and Paulsen, B. S. (2008) Pectic polysaccharides from Biophytum petersianum Klotzsch, and their activation of macrophages and dendritic cells. Glycobiology, 18 (12). pp. 1074-1084. ISSN 0959-6658

Metadata only available from this repository.


The Malian medicinal plant Biophytum petersianum Klotzsch (Oxalidaceae) is used as a treatment against various types of illnesses related to the immune system, such as joint pains, inflammations, fever, malaria, and wounds. A pectic polysaccharide obtained from a hot water extract of the aerial parts of B. petersianum has previously been reported to consist of arabinogalactans types I and II (AG-I and AG-II), probably linked to a rhamnogalacturonan backbone. We describe here further structural characteristics of the main polysaccharide fraction (BP1002) and fractions obtained by enzymatic degradations using endo -α-d-(1→4)-polygalacturonase (BP1002-I to IV). The results indicate that in addition to previously reported structures, rhamnogalacturan type II and xylogalacturonan areas appear to be present in the pectic polymer isolated from the plant. Atomic force microscopy confirmed the presence of branched structures, as well as a polydisperse nature. We further tested whether the BP1002 main fraction or the enzymatically degraded products could induce immunomodulating activity through stimulation of subsets of leukocytes. We found that macrophages and dendritic cells were activated by BP1002 fractions, while there was little response of T cells, B cells, and NK cells. The enzymatic treatment of the BP1002 main fraction gave important information on the structure-activity relations. It seems that the presence of rhamnogalacturonan type I is important for the bioactivity, as the bioactivity decreases with the decreased amounts of rhamnose, galactose, and arabinose. The demonstration of bioactivity by the plant extracts might indicate the mechanisms behind the traditional medical use of the plant. © The Author 2008. Published by Oxford University Press. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Biophytum petersianum Klotzsch Dendritic cells Macrophages Pectin Rhamnogalacturonan arabinose endo alpha dextro (1-4) polygalacturonase galactose monosaccharide nitric oxide pectic polysaccharide polygalacturonase polymer polysaccharide rhamnogalacturonan 1 rhamnogalacturonan 2 rhamnose unclassified drug xylogalacturonan animal cell culture article atomic force microscopy B lymphocyte biophytum petersianum carbohydrate analysis cell activation cell maturation controlled study dendritic cell drug activity drug isolation drug structure enzyme degradation immunomodulation leukocyte linkage analysis macrophage medicinal plant molecular weight natural killer cell nonhuman priority journal rat structure activity relation T lymphocyte Angiosperms Animals Antigen-Presenting Cells Leukocytes Lymphocytes Microscopy, Atomic Force Models, Molecular Pectins Plant Extracts Rats Rats, Inbred Strains Structure-Activity Relationship Biophytum Malia Oxalidaceae
Subjects: Q Science > QD Chemistry
Schools: School of Applied Sciences
Related URLs:
References: Language of Original Document: English Correspondence Address: Inngjerdingen, M.; Department of Pharmaceutical Chemistry, School of Pharmacy, P.O. Box 1068, Blindern N-0316 Oslo, Norway; email: Chemicals/CAS: arabinose, 147-81-9; galactose, 26566-61-0, 50855-33-9, 59-23-4; nitric oxide, 10102-43-9; polygalacturonase, 9023-92-1, 9032-75-1; rhamnose, 10485-94-6, 3615-41-6; Pectins; Plant Extracts; Polygalacturonase, EC References: Barsett, H., Paulsen, B.S., Habte, Y., Further characterization of polysaccharides in seeds from Ulmus glabra Huds (1992) Carbohydr Polym, 18, pp. 125-130; Bui, A.K., Bacic, A., Pettolino, F., Polysaccharide composition of the fruit juice of Morinda citrifolia (Noni) (2006) Phytochemistry, 67, pp. 1271-1275; Carpita, N.C., McCann, M.C., The functions of cell wall polysaccharides in composition and architecture revealed through mutations (2002) Plant Soil, 247, pp. 71-80; Chambers, R.E., Clamp, J.R., An assessment of methanolysis and other factors used in the analysis of carbohydrate-containing materials (1971) Biochem J, 125, pp. 1009-1018; Chapman, H.D., Morris, V.J., Selvendran, R.R., O'Neill, M.A., Static and dynamic light-scattering studies of pectic polysaccharides from the middle lamellae and primary cell walls of cider apples (1987) Carbohydr Res, 165, pp. 53-68; Ciucanu, I., Kerek, F., A simple and rapid method for the permethylation of carbohydrates (1984) Carbohydr Res, 131, pp. 209-217; Daas, P.J., Voragen, A.G., Schols, H.A., Study of the methyl ester distribution in pectin with endo-polygalacturonase and high-performance size-exclusion chromatography (2001) Biopolymers, 58, pp. 195-203; Diallo, D., Marston, A., Terreaux, C., Toure, Y., Paulsen, B.S., Hostettmann, K., Screening of Malian medicinal plants for antifungal, larvicidal, molluscicidal, antioxidant and radical scavenging activities (2001) Phytother Res, 15, pp. 401-406; Dourado, F., Madureira, P., Carvalho, V., Coelho, R., Coimbra, M.A., Vilanova, M., Mota, M., Gama, F.M., Purification, structure and immunobiological activity of an arabinan-rich pectic polysaccharide from the cell walls of Prunus dulcis seeds (2004) Carbohydr Res, 339, pp. 2555-2566; Dubois, M., Giles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., Colorimetric method of determination of sugars and related substances (1956) Anal Chem, 28, pp. 350-356; Guruvayoorappan, C., Kuttan, G., Effect of Biophytum sensitivum on cell-mediated immune response in mice (2007) Immunopharmacol Immunotoxicol, 29, pp. 337-350; Han, S.B., Lee, C.W., Kang, M.R., Yoon, Y.D., Kang, J.S., Lee, K.H., Yoon, W.K., Kim, H.M., Pectic polysaccharide isolated from Angelica gigas Nakai inhibits melanoma cell metastasis and growth by directly preventing cell adhesion and activating host immune functions (2006) Cancer Lett, 243, pp. 264-273; Harding, S.E., Determination of absolute molecular weights using sedimentation equilibrium analytical ultracentrifugation (1994) Methods Mol Biol, 22, pp. 75-84; Harding, S.E., Vårum, K.M., Stokke, B.T., Smidsrød, O., Molecular weight determination of polysaccharides (1991) Advances in carbohydrate analysis, 1, pp. 63-144. , White CA, ed; Hirano, M., Kiyohara, H., Matsumoto, T., Yamada, H., Structural studies of endopolygalacturonase-resistant fragments of an antiulcer pectin from the roots of Bupleurum falcatum L (1994) Carbohydr Res, 251, pp. 145-162; Hokputsa, S., Harding, S.E., Inngjerdingen, K., Jumel, K., Michaelsen, T.E., Heinze, T., Koschella, A., Paulsen, B.S., Bioactive polysaccharides from the stems of the Thai medicinal plant Acanthus ebracteatus: Their chemical and physical features (2004) Carbohydr Res, 339, pp. 753-762; Hokputsa, S., Jumel, K., Alexander, C., Harding, S.E., A comparison of molecular mass determination of hyaluronic acid using SEC/MALLS and sedimentation equilibrium (2003) Eur Biophys J, 32, pp. 450-456; Huisman, M.M., Brul, L.P., Thomas-Oates, J.E., Haverkamp, J., Schols, H.A., Voragen, A.G., The occurrence of internal (1.5)-linked arabinofuranose and arabinopyranose residues in arabinogalactan side chains from soybean pectic substances (2001) Carbohydr Res, 330, pp. 103-114; Inngjerdingen, K., Nergard, C.S., Diallo, D., Mounkoro, P.P., Paulsen, B.S., An ethnopharmacological survey of plants used for wound healing in Dogonland, Mali, West Africa (2004) J Ethnopharmacol, 92, pp. 233-244; Inngjerdingen, K.T., Coulibaly, A., Diallo, D., Michaelsen, T.E., Paulsen, B.S., A complement fixing polysaccharide from Biophytum petersianum Klotzsch, a medicinal plant from Mali, West Africa (2006) Biomacromolecules, 7, pp. 48-53; Inngjerdingen, K.T., Debes, S.C., Inngjerdingen, M., Hokputsa, S., Harding, S.E., Rolstad, B., Michaelsen, T.E., Paulsen, B.S., Bioactive pectic polysaccharides from Glinus oppositifolius (L.) Aug. DC., a Malian medicinal plant, isolation and partial characterization (2005) J Ethnopharmacol, 101, pp. 204-214; Inngjerdingen, K.T., Patel, T.R., Chen, X., Kenne, L., Allen, S., Morris, G.A., Harding, S.E., Yamada, H., Immunological and structural properties of a pectic polymer from Glinus oppositifolius (2007) Glycobiology, 17, pp. 1299-1310; Kim, J.-B., Carpita, N.C., Changes in esteri.cation of the uronic acid groups of cell wall polysaccharides during elongation of maize coleoptiles (1992) Plant Physiol, 98, pp. 646-653; Kim, J.Y., Yoon, Y.D., Ahn, J.M., Kang, J.S., Park, S.K., Lee, K., Song, K.B., Han, S.B., Angelan isolated from Angelica gigas Nakai induces dendritic cell maturation through toll-like receptor 4 (2007) Int Immunopharmacol, 7, pp. 78-87; Knutsen SH. 1991. Carrageenase production in a culture of Pseudomonas carrageenovora growing on kappa-carrageenan. In: Garcia-Reina G, Pedersen M, editors. Proceedings of a COST-48 Workshop. Universitad de Las Palmas de Gran Canaria, Las Palmas, Spain. Grand Canaria (Spain)Laue, T.M., Shah, B.D., Ridgeway, T.M., Pelletier, S.L., Computer-aided interpretation of analytical sedimentation data for proteins (1992) Analytical ultracentrifugation in biochemistry and polymer science, pp. 90-125. , Harding SE, Rowe AJ, Horton JC, eds. Cambridge UK, The Royal Society of Chemistry; Michaelsen, T.E., Gilje, A., Samuelsen, A.B., Hogasen, K., Paulsen, B.S., Interaction between human complement and a pectin type polysaccharide fraction, PMII, from the leaves of Plantago major L (2000) Scand J Immunol, 52, pp. 483-490; Miller, G.L., Use of dinitrosalicylic acid reagent for determination of reducing sugar (1959) Anal Chem, 31, pp. 426-428; Nergard, C.S., Diallo, D., Michaelsen, T.E., Malterud, K.E., Kiyohara, H., Matsumoto, T., Yamada, H., Paulsen, B.S., Isolation, partial characterisation and immunomodulating activities of polysaccharides from Vernonia kotschyana Sch. Bip. ex Walp (2004) J Ethnopharmacol, 91, pp. 141-152; Nergard, C.S., Matsumoto, T., Inngjerdingen, M., Inngjerdingen, K., Hokputsa, S., Harding, S.E., Michaelsen, T.E., Paulsen, B.S., Structural and immunological studies of a pectin and a pectic arabinogalactan from Vernonia kotschyana Sch. Bip. ex Walp. (Asteraceae) (2005) Carbohydr Res, 340, pp. 115-130; Oechslin, R., Lutz, M.V., Amado, R., Pectic substances isolated from apple cellulosic residue: Structural characterisation of a new type of rhamnogalacturonan I (2003) Carbohydr Polym, 51, pp. 301-310; O'Neill, M.A., Ishii, T., Albersheim, P., Darvill, A.G., Rhamnogalacturonan II: Structure and function of a borate cross-linked cell wall pectic polysaccharide (2004) Annu Rev Plant Biol, 55, pp. 109-139; O'Neill, M.A., York, W.S., The composition and structure of plant primary cell walls (2003) The Plant Cell Wall, pp. 1-54. , Rose JKC, editor, Oxford UK, Blackwell. p; Ovodova, R.G., Popov, S.V., Bushneva, O.A., Golovchenko, V.V., Chizhov, A.O., Klinov, D.V., Ovodov, Y.S., Branching of the galacturonan backbone of comaruman, a pectin from the marsh cinquefoil Comarum palustre L (2006) Biochemistry (Mosc), 71, pp. 538-542; Paulsen, B.S., Barsett, H., Bioactive pectic polysaccharides (2005) Adv Polym Sci, 186, pp. 69-101; Perez, S., Rodriguez-Carvajal, M.A., Doco, T., A complex plant cell wall polysaccharide: Rhamnogalacturonan II. A structure in quest of a function (2003) Biochimie, 85, pp. 109-121; Renard, C.M., Crepeau, M.J., Thibault, J.F., Glucuronic acid directly linked to galacturonic acid in the rhamnogalacturonan backbone of beet pectins (1999) Eur J Biochem, 266, pp. 566-574; Round, A.N., Rigby, N.M., MacDougall, A.J., Ring, S.G., Morris, V.J., Investigating the nature of branching in pectin by atomic force microscopy and carbohydrate analysis (2001) Carbohydr Res, 331, pp. 337-342; Sakurai, M.H., Matsumoto, T., Kiyohara, H., Yamada, H., B-cell proliferation activity of pectic polysaccharide from a medicinal herb, the roots of Bupleurum falcatum L. and its structural requirement (1999) Immunology, 97, pp. 540-547; Samuelsen, A.B., Paulsen, B.S., Wold, J.K., Otsuka, H., Kiyohara, H., Yamada, H., Knutsen, S.H., Characterization of a biologically active pectin from Plantago major L (1996) Carbohydr Polym, 30, pp. 37-44; Schuck, P., Rossmanith, P., Determination of the sedimentation coefficient distribution by least-squares boundary modeling (2000) Biopolymers, 54, pp. 328-341; Shin, K.S., Kiyohara, H., Matsumoto, T., Yamada, H., Rhamnogalacturonan II from the leaves of Panax ginseng C.A. Meyer as a macrophage Fc receptor expression-enhancing polysaccharide (1997) Carbohydr Res, 300, pp. 239-249; Singleton, V.L., Rossi, J.A.J., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents (1965) Am J Enol Viticult, 37, pp. 144-158; Strasser, G.R., Amadò, R., Pectic substances of red beet (Beta vulgaris conditiva): Part I. Structural analysis of rhamnogalacturonan I using enymatic degradation and methylation analysis (2001) Carbohydr Polym, 44, pp. 63-70; Sweet DP, Shapiro RH, Albersheim P. 1975. Quantitative analysis by various g.l.c. response-factor theories for partially methylated and partially ethylated alditol acetates. Carbohydr Res. 40:217-225Theisen, A., Johann, C., Deacon, M.P., Harding, S.E., (2000) Refractive increment data-book for polymer and biomolecular scientists, , Nottingham UK, Nottingham University Press; Vincken, J.J.P., Schols, H.A., Oomen, R.J., Beldman, G., Visser, R.G., Voragen, A.G., Pectin - The hairy thing (2003) Advances in Pectin and Pectinase Research, pp. 47-59. , Voragen AGJ, Schols H, Visser R, editors, Dordrecht Germany, Kluwer. p; Vincken, J.P., Schols, H.A., Oomen, R.J., McCann, M.C., Ulvskov, P., Voragen, A.G., Visser, R.G., If homogalacturonan were a side chain of rhamnogalacturonan I. Implications for cell wall architecture (2003) Plant Physiol, 132, pp. 1781-1789; Wagner, H., Kraus, S., News on immunologically active plant polysaccharides (2000) Bioactive Carbohydrate Polymers, pp. 1-14. , Paulsen BS, editor, Dordrecht Germany, Kluwer. p; Yamada H, Kiyohara H. 1999. Complement-activating polysaccharides from medicinal herbs. In:WAgner, editor. Immunomodulatory Agents from Plants. Basel (Switzerland): Birkhâuser. p. 161-202Yamada, H., Kiyohara, H., Immunomodulating activity of plant polysaccharide structures (2007) Comprehensive Glycoscience: From Chemistry to Systems Biology, pp. 663-694. , Kamerling JP, editor, Amsterdam: Elsevier. p; Yang, H., An, H., Li, Y., Manipulate and stretch single pectin molecules with modified molecular combing and fluid fixation techniques (2006) Eur Food Res Technol, 223, pp. 78-82
Depositing User: Gordon Morris
Date Deposited: 13 May 2011 11:37
Last Modified: 28 Aug 2021 11:06


Downloads per month over past year

Repository Staff Only: item control page

View Item View Item

University of Huddersfield, Queensgate, Huddersfield, HD1 3DH Copyright and Disclaimer All rights reserved ©