Computing and Library Services - delivering an inspiring information environment

Hydrodynamic characterization of the exopolysaccharide from the halophilic cyanobacterium Aphanothece halophytica GR02: A comparison with xanthan

Morris, Gordon, Li, P., Puaud, M., Liu, Z., Mitchell, J. R. and Harding, S. E. (2001) Hydrodynamic characterization of the exopolysaccharide from the halophilic cyanobacterium Aphanothece halophytica GR02: A comparison with xanthan. Carbohydrate Polymers, 44 (3). pp. 261-268. ISSN 0144-8617

Metadata only available from this repository.


A bacterial exopolysaccharide from Aphanothece halophytica GR02 (AH-EPS) solubilized in buffered 1 M guanidine hydrochloride, GuHCl was studied using capillary viscometry, double gap viscometry, sedimentation velocity and sedimentation equilibrium using analytical ultracentrifugation. Results from capillary viscometry indicate that the exopolysaccharide is xanthan-like in its shear thinning properties. This was confirmed by double gap viscometry. Sedimentation velocity (distribution of apparent sedimentation coefficients) confirmed that the AH-EPS solution was reasonably homogeneous and a hyper-sharp boundary indicated that AH-EPS is a rigid/extra-rigid rod type polysaccharide. Sedimentation equilibrium confirmed the high molecular weight indicated by viscosity measurements. In order to ascertain whether the solution physical properties of AH-EPS are xanthan-like under the conditions studied, all the above experiments were repeated on a similarly treated xanthan solution in the same solvent. Results indicate that AH-EPS is indeed xanthan-like in its physical properties at least in the system studied. Xanthan properties in GuHCl do not appear too different from those studied in more traditional solvents.

Item Type: Article
Uncontrolled Keywords: Bacteria Centrifugation Hydrodynamics Molecular weight Sedimentation Solubility Solvents Viscosity measurement Bacterial exopolysaccharide Guanidine hydrochloride Xanthan Polysaccharides
Subjects: Q Science > QD Chemistry
Schools: School of Applied Sciences
Related URLs:
References: Language of Original Document: English Correspondence Address: Morris, G.A.; Univ of Nottingham, Sutton Bonington, United Kingdom References: Bar, E., Visnovsky, S., Development of an algal biomatrix as a natural solution for improving soil characteristics and for land stabilization (1997) Phycologia, (Suppl.), 36, p. 5; Bar-Or, Y., Shilo, M., Characterization of marcromolecular flocculants produced by Phormidium sp. StrainJ-1 and by Anabaenopsis circularis PCC6720 (1987) Applied Environmental Microbiology, 53, pp. 2226-2230; Brock, T.D., Halophilic blue-green algae (1976) Archives in Microbiology, 107, pp. 109-111; Cesaro, A., Liut, G., Bertocchi, C., Navarini, L., Urbani, R., Physicochemical properties of the exocellular polysaccharide from Cyanospira capsulata (1990) International Journal of Biology and Macromol., 12, pp. 79-84; Choi, W.C., Yoo, S., Oh, I., Park, S.H., Characterization of an extracellular flocculating substance produced by a planktonic cyanobacterium, Anabaena sp (1998) Biotechnology Letters, 20, pp. 643-646; Cölfen, H., Harding, S.E., MSTARA and MSTARI: Interactive PC algorithms for simple, model independent evaluation of sedimentation equilibrium data (1997) European Biophysics Journal, 25, pp. 333-346; Creeth, J.M., Harding, S.E., Some observations on a new type of point average molecular weight (1982) Biochemical and Biophysical Methods, 7, pp. 25-34; Cross, M.M., Rheology of non-Newtonian fluids: A new flow equation for pseudoplastic systems (1965) Journal of Colloid Sciences, 20, pp. 417-437; De Philippis, R., Vincenzini, M., Exocellular polysaccharides from cyanobacteria and their possible applications (1998) FEMS Microbiology Reviews, 22, pp. 151-175; Dhami, R., Harding, S.E., Jones, T., Hughes, T., Mitchell, J.R., To, K., Physico-chemical studies on a commercial food grade xanthan-I. Characterisation by sedimentation velocity, sedimentation equilibrium and viscometry (1995) Carbohydrate Polymers, 27, pp. 93-99; Drews, G., Weckesser, J., Function, structure and composition of cell walls and external layers (1982) The Biology of Cyanobacteria, 19, pp. 333-357. , N.G. Carr, Whitton B.A. Oxford: Blackwell; Falchini, L., Sparvoli, E., Tomaselli, L., Effect of Nostoc (Cyanobacteria) inoculation on the structure and stability of clay soils (1996) Biology of Fertile Soils, 23, pp. 346-352; Garozzo, D., Impallomeni, G., Spina, E., Sturiale, L., The structure of the exopolysaccharide from the cyanobacterium Cyanospira capsulata (1998) Carbohydrate Research, 307, pp. 113-124; Gralen, N., (1944), PhD dissertation, University of Uppsala, SwedenGreen, A.A., The preparation of acetate and phosphate buffer solutions of known pH and ionic strength (1933) Journal of the American Chemical Society, 55, p. 2331; Harding, S.E., Some recent developments in the size and shape analysis of industrial polysaccharides in solution using sedimentation analysis in the analytical ultracentrifuge (1995) Carbohydrate Polymers, 28, pp. 227-237; Harding, S.E., The intrinsic viscosity of biological macromolecules. progress in measurement, interpretation and application to structure in dilute solutions (1998) Progress in Biophysics and Molecular Biology, 68. , Amsterdam: Elsevier. (pp. 207-262); Harding, S.E., Cölfen, H., Inversion formulae for ellipsoid of revolution macromolecular shape functions (1995) Analytical Biochemistry, 228, pp. 131-142; Harding, S.E., Berth, G., Ball, A., Mitchell, J.R., Garcia De La Torre, J., The molecular-weight distribution and conformation of citrus pectins in solutions studied by hydrodynamics (1991) Carbohydrate Polymers, 16, pp. 1-15; Huggins, M.L., The viscosity of long chain molecules IV: Dependence on concentration (1942) Journal of the American Chemical Society, 64, pp. 2716-2718; Launay, B., Cuvelier, G., Martinez-Reyes, S., (1997) Viscosity of Locust Bean, Guar and Xanthan Gum Solutions in the Newtonian Domain: A Critical Examination of the Log(ηsp)0-logc[η]0 Master Curvescarbohydrate Polymers, 34, pp. 385-395; Li, P., Liu, Z., Xu, R., Zhu, H., (2000), Submitted for publicationLiu, Z., Lin, H., A species of microalgal with useful and developable prospects - Aphanothece halophytica (1993) Sea-lake Salt & Chemical Industry, 22, pp. 16-19; Marra, M., Palmeri, A., Ballio, A., Segre, A., Slodki, M.E., Structural characterisation of the exopolysaccharide from Cyanospira capsulata (1990) Carbohydrate Research, 197, pp. 338-344; Mazor, G., Kidron, G.J., Vonshak, A., Abeliovich, A., The role of cyanobacterial exopolysaccharides in structuring desert microbial crusts (1996) FEMS Microbiology and Ecology, 21, pp. 121-130; Navarini, L., Bertocchi, C., Cesaro, A., Lapasin, R., Crescenzi, V., Rheology of aqueous solutions of an extracellular polysaccharide from Cyanospira capsulata (1990) Carbohydrate Polymers, 122, pp. 169-187; Navarini, L., Cesaro, L., Ross-Murphy, S.B., Viscoelastic properties of aqueous solutions of an exocellular polysaccharide from cyanobacteria (1992) Carbohydrate Polymers, 18, pp. 265-272; Nicolaus, B., Panico, A., Lama, L., Romano, I., Manca, M.C., De Giulio, A., Gambacorta, A., Chemical composition and production of exopolysaccharides from representative members of heterocystous and non-heterocystous cyanobacteria (1999) Phytochemistry, 52, pp. 639-647; Pavlov, G.M., Rowe, A.J., Harding, S.E., Conformation zoning of large molecules using the analytical ultracentrifuge (1997) Trends in Analytical Chemistry, 16 (7), pp. 401-405; Ralston, G., (1993) Introduction to Analytical Ultracentrifugation, , Beckman Instruments Inc., CA; Sato, T., Norisuye, T., Fujita, H., Double-stranded helix of xanthan in dilute-solution - further evidence (1984) Polymer Journal, 16, pp. 423-429; Stafford, W.F., Methods for obtaining sedimentation coefficient distributions (1992) Analytical Ultracentrifugation in Biochemistry and Polymer Science, , S.E. Harding, A.J. Rowe, & J.C. Horton. Cambridge: Royal Society of Chemistry. (chap. 20); Tanford, C., (1961) Physical Chemistry of Macromolecules, , New York: Wiley. (chap. 6); Teller, D.C., Characterisation of proteins by sedimentation equilibrium in the analytical ultracentifuge (1973) Methods in Enzymology, 27, pp. 346-441
Depositing User: Gordon Morris
Date Deposited: 13 May 2011 11:05
Last Modified: 28 Aug 2021 11:06


Downloads per month over past year

Repository Staff Only: item control page

View Item View Item

University of Huddersfield, Queensgate, Huddersfield, HD1 3DH Copyright and Disclaimer All rights reserved ©