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Hydrodynamic characterization of the exopolysaccharide from the halophilic cyanobacterium Aphanothece halophytica GR02: A comparison with xanthan

Morris, Gordon, Li, P., Puaud, M., Liu, Z., Mitchell, J. R. and Harding, S. E. (2001) Hydrodynamic characterization of the exopolysaccharide from the halophilic cyanobacterium Aphanothece halophytica GR02: A comparison with xanthan. Carbohydrate Polymers, 44 (3). pp. 261-268. ISSN 0144-8617

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A bacterial exopolysaccharide from Aphanothece halophytica GR02 (AH-EPS) solubilized in buffered 1 M guanidine hydrochloride, GuHCl was studied using capillary viscometry, double gap viscometry, sedimentation velocity and sedimentation equilibrium using analytical ultracentrifugation. Results from capillary viscometry indicate that the exopolysaccharide is xanthan-like in its shear thinning properties. This was confirmed by double gap viscometry. Sedimentation velocity (distribution of apparent sedimentation coefficients) confirmed that the AH-EPS solution was reasonably homogeneous and a hyper-sharp boundary indicated that AH-EPS is a rigid/extra-rigid rod type polysaccharide. Sedimentation equilibrium confirmed the high molecular weight indicated by viscosity measurements. In order to ascertain whether the solution physical properties of AH-EPS are xanthan-like under the conditions studied, all the above experiments were repeated on a similarly treated xanthan solution in the same solvent. Results indicate that AH-EPS is indeed xanthan-like in its physical properties at least in the system studied. Xanthan properties in GuHCl do not appear too different from those studied in more traditional solvents.

Item Type: Article
Uncontrolled Keywords: Bacteria Centrifugation Hydrodynamics Molecular weight Sedimentation Solubility Solvents Viscosity measurement Bacterial exopolysaccharide Guanidine hydrochloride Xanthan Polysaccharides
Subjects: Q Science > QD Chemistry
Schools: School of Applied Sciences
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Depositing User: Gordon Morris
Date Deposited: 13 May 2011 11:05
Last Modified: 06 Apr 2018 12:00


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