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Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide

Morris, Gordon, Butler, S. N. G., Foster, T. J., Jumel, K. and Harding, S. E. (1999) Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide. Progress in Colloid and Polymer Science, 113. pp. 205-208. ISSN 0340-255X

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Relatively little has been published on the ultracentrifuge behaviour of macromolecular solutions at elevated temperature (> 40°C). In this study we look at the sedimentation velocity behaviour of one particular food grade polyuronide - pectin - from 20°C to 60°C in a specially adapted Model E ultracentrifuge. Reduced specific viscosity measurements were also determined over the same temperature range. A small decrease in the reduced viscosity, together with a similar increase in s20,w, suggests that the pectin chain is more flexible at elevated temperatures, but that the overall molecular integrity remains intact. © Springer-Verlag 1999.

Item Type: Article
Uncontrolled Keywords: Beckman Model E adaptation Size exclusion chromatography-multiangle laser light scattering Viscometry
Subjects: Q Science > QD Chemistry
Schools: School of Applied Sciences
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References: Language of Original Document: English Correspondence Address: Morris, G.A.; NCMH Unit, School of Biological Sciences, University of Nottingham, Sutton Bonington, Leics. LE12 5RD, United Kingdom; email: References: Tombs, M.P., Harding, S.E., (1998) An Introduction to Polysaccharide Biotechnology, , Taylor and Francis, London; Green, A.A., (1933) J Am Chem Soc, 25, p. 2331; Pavlov, G.M., (1997) Eur Biophys J, 25, p. 385; (1974) Beckman Spinco Service Manual, , Beckman Instruments, Palo Alto, Calif; Van Holde, K.E., (1985) Physical Bochem-istry, 2nd Edn., , Prentice-Hall, Englewood Cliffs, NJ; Jumel, K., Browne, P., Kennedy, J.F., (1992) Laser Light Scattering in Biochemistry, , Harding SE, Sattelle DB, Bloomfeld VA (eds) chap 2. Royal Society of Chemistry, Cambridge; Jumel, K., (1994), Ph D Thesis, University of NottinghamWyatt, P.J., (1992) Laser Light Scattering in Biochemistry, , Harding SE, Sattelle DB, Bloomfeld VA (eds) chap 3. Royal Society of Chemistry, Cambridge; Harding, S.E., Berth, G., Ball, A., Mitchell, J.R., Garcia De La Torre, J., (1991) Carbohydr Polym, 16, p. 1
Depositing User: Gordon Morris
Date Deposited: 19 May 2011 12:00
Last Modified: 28 Aug 2021 11:06


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