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Alginate-Loaded Liposomes Can Protect Encapsulated Alkaline Phosphatase Functionality When Exposed to Gastric pH

Smith, Alan M., Jaime-Fonseca, Monica R., Grover, Liam M. and Bakalis, Serafim (2010) Alginate-Loaded Liposomes Can Protect Encapsulated Alkaline Phosphatase Functionality When Exposed to Gastric pH. Journal of Agricultural and Food Chemistry, 58 (8). pp. 4719-4724. ISSN 0021-8561

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    Abstract

    The present study has evaluated the potential of alginate-loaded liposomes as a vehicle for the oral delivery of bioactive proteins. The vesicles were prepared from the phospholipid dipalmatoyl
    phosphatidylcholine using a simple dry film hydration technique. Alkaline phosphatase (ALP) was used as a model bioactive protein, which was encapsulated in alginate-loaded liposomes and
    conventional liposomes. These vesicles were subject to physical characterization and stability
    analysis at varying pH values. The enzymatic activity of the ALP was evaluated following exposure to simulated gastric pH. The alginate-loaded liposomes were typically of the order of 10 μm; however, there was evidence of vesicle aggregation thought to be due to alginate present on the surface of the vesicles. The typical size of the aggregated vesicles was ∼30 μm. The enzyme
    activity of APL following 2 h of exposure to simulated gastric pH was maintained at a significantly higher level when encapsulated in the alginate-loaded liposomes as compared to ALP loaded in conventional liposomes [80% as compared to 55% (p < 0.05), respectively].

    Item Type: Article
    Subjects: Q Science > Q Science (General)
    Q Science > QD Chemistry
    R Medicine > RS Pharmacy and materia medica
    Schools: School of Applied Sciences
    Depositing User: Alan Smith
    Date Deposited: 21 Mar 2011 16:11
    Last Modified: 21 Mar 2011 16:11
    URI: http://eprints.hud.ac.uk/id/eprint/9877

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