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Polysaccharide determination in protein/polysaccharide mixtures for phase-diagram construction

Agbenorhevi, Jacob K. and Kontogiorgos, Vassilis (2010) Polysaccharide determination in protein/polysaccharide mixtures for phase-diagram construction. Carbohydrate Polymers, 81 (4). pp. 849-854. ISSN 01448617

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    Abstract

    The effect of protein removal from protein–polysaccharide mixtures on the determination of total
    sugar concentration for phase-diagram construction was explored by response surface methodology.
    Phenol–sulphuric acid method was used to estimate the total sugar concentration whereas phase separation
    and microstructure of biopolymers in the mixtures were probed by means of fluorescent microscopy.
    All the factors studied (trifluoroacetic acid (TFA) volume, protein concentration, precipitation time) had
    significant influence on the polysaccharide recovery from the mixtures. The overall pattern of protein
    precipitation with TFA was also found to highly depend on protein–time interactions. Images taken at different
    mixture concentrations and with two different filter sets revealed a phase-separated system with
    the guar gum domains surrounded by a continuous protein phase. Experimental measurements as well as
    microscopical evidence suggests that, for greater than 60% polysaccharide recovery (±6% error), the protein
    content in the mixtures should be <∼5.5% (w/v) with less than 0.5mL TFA combined with more than
    30 minrest under the influence of TFA. The results of the present investigationshowedthatwhenattempting
    to construct phase diagram of mixed biopolymer systems the equilibrium phases must be diluted to
    a protein content <∼5% (w/v) before TFA treatment if the maximum polysaccharide concentration is to
    be determined.

    Item Type: Article
    Subjects: Q Science > QD Chemistry
    Schools: School of Applied Sciences
    Related URLs:
    Depositing User: Vasileios Kontogioros
    Date Deposited: 09 Nov 2010 15:15
    Last Modified: 31 Mar 2011 04:58
    URI: http://eprints.hud.ac.uk/id/eprint/8967

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