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Expression of the chloride channel CLC-K in human airway epithelial cells

Mummery, Jennifer L., Killey, Jennifer and Linsdell, Paul (2005) Expression of the chloride channel CLC-K in human airway epithelial cells. Canadian Journal of Physiology and Pharmacology, 83 (12). p. 1123. ISSN 00084212

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    Abstract

    Airway submucosal gland function is severely disrupted in cystic fibrosis (CF), as a result of genetic mutation
    of the cystic fibrosis transmembrane conductance regulator (CFTR), an apical membrane Cl– channel. To identify other
    Cl– channel types that could potentially substitute for lost CFTR function in these cells, we investigated the functional and
    molecular expression of Cl– channels in Calu-3 cells, a human cell line model of the submucosal gland serous cell. Whole
    cell patch clamp recording from these cells identified outwardly rectified, pH- and calcium-sensitive Cl– currents that resemble
    those previously ascribed to ClC-K type chloride channels. Using reverse transcription – polymerase chain reaction,
    we identified expression of mRNA for ClC-2, ClC-3, ClC-4, ClC-5, ClC-6, ClC-7, ClC-Ka, and ClC-Kb, as well as the
    common ClC-K channel b subunit barttin. Western blotting confirmed that Calu-3 cells express both ClC-K and barttin
    protein. Thus, Calu-3 cells express multiple members of the ClC family of Cl– channels that, if also expressed in native
    submucosal gland serous cells within the CF lung, could perhaps act to partially substitute lost CFTR function. Furthermore,
    this work represents the first evidence for functional ClC-K chloride channel expression within the lung.

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    Item Type: Article
    Additional Information: © 2005 NRC Canada
    Uncontrolled Keywords: chloride channel, epithelial transport, airway, cystic fibrosis.
    Subjects: Q Science > Q Science (General)
    Q Science > QD Chemistry
    Schools: School of Human and Health Sciences
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    by multifunctional Ca2+/calmodulin-dependent protein ki-

    Depositing User: Sara Taylor
    Date Deposited: 01 May 2008 15:09
    Last Modified: 28 Jul 2010 19:22
    URI: http://eprints.hud.ac.uk/id/eprint/704

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