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A collaborative exercise on DNA methylation-based body fluid typing

Jung, Sang-Eun, Cho, Sohee, Antunes, Joana, Gomes, Iva, Uchimoto, Mari L., Oh, Yu Na, Giacomo, Lisa Di, Schneider, Peter M., Park, Min Sun, van der Meer, Dieudonne J., Williams, Graham, McCord, Bruce, Ahn, Hee-Jung, Choi, Dong Ho, Lee, Yang-Han, Lee, Soong Deok and Lee, Hwan Young (2016) A collaborative exercise on DNA methylation-based body fluid typing. Electrophoresis, 37 (21). pp. 2759-2766. ISSN 0173-0835

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Abstract

A collaborative exercise on DNA methylation-based body fluid identification was conducted by seven laboratories. For this project, a multiplex methylation SNaPshot reaction composed of seven CpG markers was used for the identification of 4 body fluids, including blood, saliva, semen and vaginal fluid. A total of 30 specimens were prepared and distributed to participating laboratories after thorough testing. The required experiments included four increasingly complex tasks: 1) capillary electrophoresis of a purified single-base extension reaction product, 2) multiplex PCR of bisulfite-modified DNA, 3) bisulfite conversion of genomic DNA and 4) extraction of genomic DNA from body fluid samples. In tasks 2, 3 and 4, one or more mixtures were analyzed, and specimens containing both known and unknown body fluid sources were used. Six of the laboratories generated consistent body fluid typing results for specimens of bisulfite converted DNA and genomic DNA. One laboratory failed to set up appropriate conditions for capillary analysis of reference single-base extension products. In general, variation in the values obtained for DNA methylation analysis between laboratories increased with the complexity of the required experiments. However, all laboratories concurred on the interpretation of the DNA methylation profiles produced. Although the establishment of interpretational guidelines on DNA methylation-based body fluid identification has yet to be performed, this study supports the addition of DNA methylation profiling to forensic body fluid typing.

Item Type: Article
Subjects: Q Science > Q Science (General)
Schools: School of Applied Sciences
School of Applied Sciences > Forensic Biology Research Group
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Depositing User: Elizabeth Boulton
Date Deposited: 31 Aug 2016 14:29
Last Modified: 05 Dec 2016 10:56
URI: http://eprints.hud.ac.uk/id/eprint/29310

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