Nep, Elijah I, Carnachan, Susan, Ngwuluka, Ndidi, Kontogiorgos, Vassilis, Morris, Gordon, Sims, Ian and Smith, Alan M. (2016) Structural characterisation and rheological properties of a polysaccharide from sesame leaves (Sesamum radiatum Schumach. & Thonn.). Carbohydrate Polymers, 152. pp. 541-547. ISSN 0144-8617
- Accepted Version
Restricted to Repository staff only until 11 July 2017.
A polysaccharide from the leaves of Sesamum radiatum was extracted by maceration in deionized water followed by ethanol precipitation then chemically and physically characterised. Monosaccharide composition and linkages were determined by high performance anion exchange chromatography (HPAEC), gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy respectively. Sesamum gum was composed of glucuronic acid, mannose, galactose, and xylose with trace quantities of glucose, rhamnose and arabinose. Proton and 13C NMR spectroscopy, and linkage analysis revealed a glucuronomannan based structure comprising a backbone of 4)--D-GlcpA-(12)--D-Manp-(1 with side-chains of galactose and xylose. Hydrated sesamum gum displayed temperature independent viscoelastic properties with no thermal hysteresis. Intrinsic viscosity was determined to be 3.31 and 4.40 dLg-1 in 0.1 M NaCl and deionised water respectively, while the critical concentration was determined to be 0.1 % w/v. The characterisation performed in this study will help direct potential applications of this material in foods and pharmaceuticals.
|Subjects:||Q Science > Q Science (General)
Q Science > QD Chemistry
Q Science > QK Botany
R Medicine > RS Pharmacy and materia medica
|Schools:||School of Applied Sciences|
|Depositing User:||Alan Smith|
|Date Deposited:||21 Jul 2016 15:52|
|Last Modified:||09 Aug 2016 14:25|
Downloads per month over past year
Repository Staff Only: item control page