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Penetration of normal, damaged and diseased skin--an in vitro study on dendritic core-multishell nanotransporters

Alnasif, N., Zoschke, C., Fleige, E., Brodwolf, R., Boreham, A., Ruhl, E., Eckl, K.M., Merk, H.F., Hennies, Hans C., Alexiev, U., Haag, R., Küchler, S. and Schäfer-Korting, M. (2014) Penetration of normal, damaged and diseased skin--an in vitro study on dendritic core-multishell nanotransporters. Journal of Controlled Release, 185. pp. 45-50. ISSN 0168-3659

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Abstract

A growing intended or accidental exposure to nanoparticles asks for the elucidation of potential toxicity linked to the penetration of normal and lesional skin. We studied the skin penetration of dye-tagged dendritic core-multishell (CMS) nanotransporters and of Nile red loaded CMS nanotransporters using fluorescence microscopy. Normal and stripped human skin ex vivo as well as normal reconstructed human skin and in vitro skin disease models served as test platforms. Nile red was delivered rapidly into the viable epidermis and dermis of normal skin, whereas the highly flexible CMS nanotransporters remained solely in the stratum corneum after 6h but penetrated into deeper skin layers after 24h exposure. Fluorescence lifetime imaging microscopy proved a stable dye-tag and revealed striking nanotransporter-skin interactions. The viable layers of stripped skin were penetrated more efficiently by dye-tagged CMS nanotransporters and the cargo compared to normal skin. Normal reconstructed human skin reflected the penetration of Nile red and CMS nanotransporters in human skin and both, the non-hyperkeratotic non-melanoma skin cancer and hyperkeratotic peeling skin disease models come along with altered absorption in the skin diseases.

Item Type: Article
Additional Information: Unmapped bibliographic data: ST - Penetration of normal, damaged and diseased skin--an in vitro study on dendritic core-multishell nanotransporters [Field not mapped to EPrints] AD - Institute for Pharmacy (Pharmacology and Toxicology), Freie Universitat Berlin, Berlin, Germany. [Field not mapped to EPrints] AN - 24727058 [Field not mapped to EPrints]
Uncontrolled Keywords: Drug Carriers/analysis/*metabolism, Nanoparticles/analysis/*metabolism , Nanotoxicology
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Schools: School of Applied Sciences
Depositing User: Hans Hennies
Date Deposited: 06 Jul 2016 11:04
Last Modified: 29 Jul 2016 13:02
URI: http://eprints.hud.ac.uk/id/eprint/28764

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