Search:
Computing and Library Services - delivering an inspiring information environment

Maturation of the unusual single-cysteine (XXXCH) mitochondrialc-type cytochromes found in trypanosomatids must occur through a novel biogenesis pathway

Allen, James W. A., Ginger, Michael L. and Ferguson, Stuart J. (2004) Maturation of the unusual single-cysteine (XXXCH) mitochondrialc-type cytochromes found in trypanosomatids must occur through a novel biogenesis pathway. Biochemical Journal, 383 (3). pp. 537-542. ISSN 0264-6021

Metadata only available from this repository.

Abstract

The c-type cytochromes are characterized by the covalent attachment of haem to the polypeptide via thioether bonds formed from haem vinyl groups and, normally, the thiols of two cysteines in a CXXCH motif. Intriguingly, the mitochondrial cytochromes c and c1 from two euglenids and the Trypanosomatidae contain only a single cysteine within the haem-binding motif (XXXCH). There are three known distinct pathways by which c-type cytochromes are matured post-translationally in different organisms. The absence of genes encoding any of these c-type cytochrome biogenesis machineries is established here by analysis of six trypanosomatid genomes, and correlates with the presence of single-cysteine cytochromes c and c1. In contrast, we have identified a comprehensive catalogue of proteins required for a typical mitochondrial oxidative phosphorylation apparatus. Neither spontaneous nor catalysed maturation of the single-cysteine Trypanosoma brucei cytochrome c occurred in Escherichia coli. However, a CXXCH variant was matured by the E. coli cytochrome c maturation machinery, confirming the proposed requirement of the latter for two cysteines in the haem-binding motif and indicating that T. brucei cytochrome c can accommodate a second cysteine in a CXXCH motif. The single-cysteine haem attachment conserved in cytochromes c and c1 of the trypanosomatids is suggested to be related to their cytochrome c maturation machinery, and the environment in the mitochondrial intermembrane space. Our genomic and biochemical studies provide very persuasive evidence that the trypanosomatid mitochondrial cytochromes c are matured by a novel biogenesis system.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Schools: School of Applied Sciences
Related URLs:
Depositing User: Michael Ginger
Date Deposited: 10 May 2016 08:42
Last Modified: 10 May 2016 08:42
URI: http://eprints.hud.ac.uk/id/eprint/28275

Downloads

Downloads per month over past year

Repository Staff Only: item control page

View Item View Item

University of Huddersfield, Queensgate, Huddersfield, HD1 3DH Copyright and Disclaimer All rights reserved ©