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Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology

Hayes, Polly, Varga, Vladimir, Olego-Fernandez, Sofia, Sunter, Jack, Ginger, Michael L. and Gull, Keith (2014) Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology. The Journal of Cell Biology, 206 (3). pp. 377-384. ISSN 0021-9525

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Abstract

Individual eukaryotic microbes, such as the kinetoplastid parasite Trypanosoma brucei, have a defined size, shape, and form yet transition through life cycle stages, each having a distinct morphology. In questioning the structural processes involved in these transitions, we have identified a large calpain-like protein that contains numerous GM6 repeats (ClpGM6) involved in determining T. brucei cell shape, size, and form. ClpGM6 is a cytoskeletal protein located within the flagellum along the flagellar attachment zone (FAZ). Depletion of ClpGM6 in trypomastigote forms produces cells with long free flagella and a shorter FAZ, accompanied by repositioning of the basal body, the kinetoplast, Golgi, and flagellar pocket, reflecting an epimastigote-like morphology. Hence, major changes in microbial cell form can be achieved by simple modulation of one or a few proteins via coordinated association and positioning of membrane and cytoskeletal components.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Schools: School of Applied Sciences
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Depositing User: Michael Ginger
Date Deposited: 09 May 2016 12:55
Last Modified: 01 Dec 2016 05:39
URI: http://eprints.hud.ac.uk/id/eprint/28241

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