Computing and Library Services - delivering an inspiring information environment

Effect of Transforming Growth Factor-β3 on mono and multilayer chondrocytes

Sefat, Farshid, Youseffi, Mansour, Khaghani, Seyed Ali, Soon, Chin Fhung and Javid, Farideh A. (2016) Effect of Transforming Growth Factor-β3 on mono and multilayer chondrocytes. Cytokine, 83. pp. 118-126. ISSN 1043-4666

[img] PDF - Accepted Version
Restricted to Repository staff only until July 2017.

Download (1MB)


Articular cartilage is an avascular and flexible connective tissue found in joints. It produces a cushioning effect at the joints and provides low friction to protect the ends of the bones from wear and tear/damage. It has poor repair capacity and any injury can result pain and loss of mobility. Transforming growth factor-beta (TGF-β), a cytokine superfamily, regulates cell function, including differentiation and proliferation. Although the function of the TGF-βs in various cell types has been investigated, their function in cartilage repair is as yet not fully understood. The effect of TGF-β3 in biological regulation of primary chondrocyte was investigated in this work. TGF-β3 provide fibroblastic morphology to chondrocytes and therefore overall reduction in cell proliferation was observed. The length of the cells supplemented with TGF-β3 were larger than the cells without TGF-β3 treatment. This was caused by the fibroblast like cells (dedifferentiated chondrocytes) which occupied larger areas compared to cells without TGF-β3 addition. The healing process of the model wound closure assay of chondrocyte multilayer was slowed down by TGF-β3, and this cytokine negatively affected the strength of chondrocyte adhesion to the cell culture surface.

Item Type: Article
Subjects: R Medicine > RS Pharmacy and materia medica
Schools: School of Applied Sciences
Related URLs:
Depositing User: Cherry Edmunds
Date Deposited: 26 Apr 2016 12:34
Last Modified: 18 May 2016 15:15


Downloads per month over past year

Repository Staff Only: item control page

View Item View Item

University of Huddersfield, Queensgate, Huddersfield, HD1 3DH Copyright and Disclaimer All rights reserved ©