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Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation

Keates, Tracy, Cooper, Christopher D.O., Savitsky, Pavel, Allerston, Charles K., Phillips, Claire, Hammarström, Martin, Daga, Neha, Berridge, Georgina, Mahajan, Pravin, Burgess-Brown, Nicola A., Müller, Susanne, Gräslund, Susanne and Gileadi, Opher (2012) Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation. New Biotechnology, 29 (5). pp. 515-525. ISSN 1876-4347

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Abstract

The generation of affinity reagents to large numbers of human proteins depends on the ability to express the target proteins as high-quality antigens. The Structural Genomics Consortium (SGC) focuses on the production and structure determination of human proteins. In a 7-year period, the SGC has deposited crystal structures of >800 human protein domains, and has additionally expressed and purified a similar number of protein domains that have not yet been crystallised. The targets include a diversity of protein domains, with an attempt to provide high coverage of protein families. The family approach provides an excellent basis for characterising the selectivity of affinity reagents. We present a summary of the approaches used to generate purified human proteins or protein domains, a test case demonstrating the ability to rapidly generate new proteins, and an optimisation study on the modification of >70 proteins by biotinylation in vivo. These results provide a unique synergy between large-scale structural projects and the recent efforts to produce a wide coverage of affinity reagents to the human proteome.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Schools: School of Applied Sciences
Depositing User: Christopher Cooper
Date Deposited: 23 Nov 2015 12:15
Last Modified: 04 Dec 2016 00:00
URI: http://eprints.hud.ac.uk/id/eprint/26494

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