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Structural insights into the autoregulation and cooperativity of the human transcription factor Ets-2

Newman, Joseph A., Cooper, Christopher D.O., Aitkenhead, Hazel and Gileadi, Opher (2015) Structural insights into the autoregulation and cooperativity of the human transcription factor Ets-2. The Journal of Biological Chemistry, 290 (13). pp. 8539-8549. ISSN 1083-351X

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Ets-2, like its closely related homologue Ets-1, is a member of the Ets family of DNA binding transcription factors. Both proteins are subject to multiple levels of regulation of their DNA binding and transactivation properties. One such regulatory mechanism is the presence of an autoinhibitory module, which in Ets-1 allosterically inhibits the DNA binding activity. This inhibition can be relieved by interaction with protein partners or cooperative binding to closely separated Ets binding sites in a palindromic arrangement. In this study we describe the 2.5 Å resolution crystal structure of a DNA complex of the Ets-2 Ets domain. The Ets domain crystallized with two distinct species in the asymmetric unit, which closely resemble the autoinhibited and DNA bound forms of Ets-1. This discovery prompted us to re-evaluate the current model for the autoinhibitory mechanism and the structural basis for cooperative DNA binding. In contrast to Ets-1, in which the autoinhibition is caused by a combination of allosteric and steric mechanisms, we were unable to find clear evidence for the allosteric mechanism in Ets-2. We also demonstrated two possibly distinct types of cooperative binding to substrates with Ets binding motifs separated by four and six base pairs and suggest possible molecular mechanisms for this behavior.

Item Type: Article
Additional Information: J.A.Newman and C.D.O.Cooper are first co-authors/ equal contribution
Subjects: Q Science > QH Natural history > QH301 Biology
Schools: School of Applied Sciences
Depositing User: Christopher Cooper
Date Deposited: 23 Nov 2015 11:52
Last Modified: 19 Dec 2015 13:00


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