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Comparative characterisation of two Rhodococcus species as potential biocatalysts for ammonium acrylate production

Webster, N.A., Ramsden, D. K. and Hughes, J. (2001) Comparative characterisation of two Rhodococcus species as potential biocatalysts for ammonium acrylate production. Biotechnology Letters, 23 (2). pp. 95-101. ISSN 0141-5492

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Abstract

Two Rhodococcal isolates, one possessing a nitrile hydratase and an amidase enzyme, the other an aliphatic nitrilase enzyme have been isolated. The kinetic constants for the enzymes in each isolate have been determined. This data coupled with stability tests indicate that Rhodococcus ruber NCIMB 40757, the nitrilase containing organism,should be an excellent biocatalyst for the commercial production of ammonium acrylate. This is confirmed by a fed-batch bioconversion to produce 5.7 M ammonium acrylate.

Item Type: Article
Subjects: T Technology > TP Chemical technology
Schools: School of Applied Sciences
Depositing User: Catherine Parker
Date Deposited: 07 Nov 2008 13:07
Last Modified: 07 Nov 2008 13:07
URI: http://eprints.hud.ac.uk/id/eprint/2585

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