Webster, N.A., Ramsden, D. K. and Hughes, J. (2001) Comparative characterisation of two Rhodococcus species as potential biocatalysts for ammonium acrylate production. Biotechnology Letters, 23 (2). pp. 95-101. ISSN 0141-5492Metadata only available from this repository.
Two Rhodococcal isolates, one possessing a nitrile hydratase and an amidase enzyme, the other an aliphatic nitrilase enzyme have been isolated. The kinetic constants for the enzymes in each isolate have been determined. This data coupled with stability tests indicate that Rhodococcus ruber NCIMB 40757, the nitrilase containing organism,should be an excellent biocatalyst for the commercial production of ammonium acrylate. This is confirmed by a fed-batch bioconversion to produce 5.7 M ammonium acrylate.
|Subjects:||T Technology > TP Chemical technology|
|Schools:||School of Applied Sciences|
|Depositing User:||Catherine Parker|
|Date Deposited:||07 Nov 2008 13:07|
|Last Modified:||07 Nov 2008 13:07|
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