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Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods

Harding, S. E., Abdelhameed, A. S., Gillis, R. B., Morris, Gordon and Adams, G. G. (2015) Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods. Methods in Molecular Biology, 1331. pp. 211-227. ISSN 1064-3745

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Abstract

Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), and capillary viscometry—intrinsic viscosity measurement. The chapter highlights the general principle of these five methods, describes experimental details, and specifies advances in the last years.

Item Type: Article
Subjects: Q Science > QD Chemistry
Schools: School of Applied Sciences
Related URLs:
Depositing User: Gordon Morris
Date Deposited: 15 Sep 2015 10:07
Last Modified: 15 Sep 2015 10:07
URI: http://eprints.hud.ac.uk/id/eprint/25575

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