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Structural characterisation of a perdeuteriomethylated exoploysaccharide by NMR spectroscopy: characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. Bulgaricus EU23

Harding, Lindsay P., Marshall, Valerie M., Elvin, M., Gu, Y. and Laws, Andrew P. (2003) Structural characterisation of a perdeuteriomethylated exoploysaccharide by NMR spectroscopy: characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. Bulgaricus EU23. Carbohydrate Research, 338 (1). pp. 61-67. ISSN 0008-6215

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Abstract

The exopolysaccharide (EPS) from Lactobacillus delbrueckii subsp. bulgaricus EU23 was perdeuteriomethylated and the perdeuteriomethylated EPS (pdm-EPS) purified by elution from a C18 Sep-Pak cartridge. Both 1D and 2D NMR spectra were recorded for the pdm-EPS and these were interpreted to provide assignments for the individual 1H and 13C resonances of the sugar residues of the repeating unit. Using a combination of the results from monomer analysis and linkage analysis of the native EPS and the ROESY and HMBC NMR spectra of the pdm-EPS the following structure has been determined for the repeating unit:

Item Type: Article
Additional Information: UoA 18 (Chemistry) Copyright © 2007 Elsevier B.V. All rights reserved. ScienceDirect® is a registered trademark of Elsevier B.V.
Subjects: Q Science > QD Chemistry
Schools: School of Applied Sciences
School of Applied Sciences > Biomolecular Sciences Research Centre
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Depositing User: Briony Heyhoe
Date Deposited: 13 Jul 2007
Last Modified: 03 Dec 2016 00:31
URI: http://eprints.hud.ac.uk/id/eprint/255

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