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A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw

Pensupa, Nattha, Jin, Meng, Kokolski, Matt, Archer, David B. and Du, Chenyu (2013) A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw. Bioresource Technology, 149. pp. 261-267. ISSN 09608524

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Abstract

This paper reports a solid-state fungal fermentation-based pre-treatment strategy to convert wheat straw into a fermentable hydrolysate. Aspergillus niger was firstly cultured on wheat straw for production of cellulolytic enzymes and then the wheat straw was hydrolyzed by the enzyme solution into a fermentable hydrolysate. The optimum moisture content and three wheat straw modification methods were explored to improve cellulase production. At a moisture content of 89.5%, 10.2 ± 0.13 U/g cellulase activity was obtained using dilute acid modified wheat straw. The addition of yeast extract (0.5% w/v) and minerals significantly improved the cellulase production, to 24.0 ± 1.76 U/g. The hydrolysis of the fermented wheat straw using the fungal culture filtrate or commercial cellulase Ctec2 was performed, resulting in 4.34 and 3.13 g/L glucose respectively. It indicated that the fungal filtrate harvested from the fungal fermentation of wheat straw contained a more suitable enzyme mixture than the commercial cellulase.

Item Type: Article
Subjects: T Technology > TP Chemical technology
Schools: School of Applied Sciences
Related URLs:
Depositing User: Chenyu Du
Date Deposited: 05 Mar 2015 15:57
Last Modified: 30 Nov 2016 17:38
URI: http://eprints.hud.ac.uk/id/eprint/23386

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