Pensupa, Nattha, Jin, Meng, Kokolski, Matt, Archer, David B. and Du, Chenyu (2013) A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw. Bioresource Technology, 149. pp. 261-267. ISSN 09608524
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Available under License Creative Commons Attribution.
This paper reports a solid-state fungal fermentation-based pre-treatment strategy to convert wheat straw into a fermentable hydrolysate. Aspergillus niger was firstly cultured on wheat straw for production of cellulolytic enzymes and then the wheat straw was hydrolyzed by the enzyme solution into a fermentable hydrolysate. The optimum moisture content and three wheat straw modification methods were explored to improve cellulase production. At a moisture content of 89.5%, 10.2 ± 0.13 U/g cellulase activity was obtained using dilute acid modified wheat straw. The addition of yeast extract (0.5% w/v) and minerals significantly improved the cellulase production, to 24.0 ± 1.76 U/g. The hydrolysis of the fermented wheat straw using the fungal culture filtrate or commercial cellulase Ctec2 was performed, resulting in 4.34 and 3.13 g/L glucose respectively. It indicated that the fungal filtrate harvested from the fungal fermentation of wheat straw contained a more suitable enzyme mixture than the commercial cellulase.
|Subjects:||T Technology > TP Chemical technology|
|Schools:||School of Applied Sciences|
|Depositing User:||Chenyu Du|
|Date Deposited:||05 Mar 2015 15:57|
|Last Modified:||04 Nov 2015 19:51|
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