Computing and Library Services - delivering an inspiring information environment

Identification of angiotensin I in several vertebrate species: its structural and functional evolution

Takei, Yoshio, Joss, Jean M.P., Kloas, Werner and Rankin, J. Cliff (2004) Identification of angiotensin I in several vertebrate species: its structural and functional evolution. General and Comparative Endocrinology, 135 (3). pp. 286-292. ISSN 0016-6480

[img] PDF
Restricted to Registered users only

Download (259kB)


In order to delineate further the molecular evolution of the renin–angiotensin system in vertebrates, angiotensin I (ANG I) has been isolated after incubation of plasma and kidney extracts of emu (Dromiceus novaehollandiae), axolotl (Ambystoma mexicanum), and sea lamprey (Petromyzon marinus). The identified sequences were [Asp1, Val5, Asn9] ANG I in emu, [Asp1, Val5, His9] ANG I in axolotl, and [Asn1, Val5, Thr9] ANG I in sea lamprey. These results confirmed the previous findings that tetrapods have Asp and fishes including cyclostomes have Asn at the N-terminus, and that the amino acid residue at position 9 of ANG I was highly variable but, those at other positions were well conserved among different species. Since Asp and Asn are convertible during incubation, angiotensinogen sequences were searched in the genome and/or EST database to determine the N-terminal amino acid residue from the gene. The screening detected 12 tetrapod (10 mammalian, one avian, and one amphibian) and seven teleostean angiotensinogen sequences. Among them, all tetrapods have [Asp1] ANG except for Xenopus, and all teleosts have [Asn1] ANG, thereby confirming the above rule. Comparison of the vasopressor activity in the eel revealed that [Asn1] ANG I and II were more potent than [Asp1] peptides, which was opposite to the previous results in mammals and birds, in which [Asp1] ANG I and II were more potent. Collectively, the present results support the general rule that tetrapods have [Asp1] ANG and fishes including cyclostomes have [Asn1] ANG. However, an aquatic anuran (Xenopus) has [Asn1] ANG in its gene despite another aquatic urodele (axolotl) has [Asp1] ANG. From the functional viewpoint, homologous [Asn1] ANG was more potent in fish as is homologous [Asp1] ANG in tetrapods, suggesting that ANG II molecule has undergone co-evolution with its receptor during vertebrate phylogeny.

Item Type: Article
Additional Information: © 2003 Published by Elsevier Inc.
Uncontrolled Keywords: Renin–angiotensin system; Emu, Dromiceus novaehollandiae; Axolotl, Ambystoma mexicanum; Sea lamprey, Petromyzon marinus; Molecular evolution; Vasopressor effect in eel
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Schools: School of Applied Sciences

Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J., 1990.
Basic local alignment search tool. J. Mol. Biol. 215, 403–410.
Balment, R.J., Warne, J.M., Takei, Y., 2003. Isolation, synthesis and
biological activity of flounder [Asn1, Ile5, Thr9] angiotensin I. Gen.
Comp. Endocrinol. 130, 92–98.
Boucher, R., Menard, J., Genest, J., 1967. A micromethod for
measurement of renin in the plasma and kidney of rats. Can. J.
Physiol. Pharmacol. 45, 881–890.
Brown, J.A., Balment, R.J., 1997. Teleost renal function: regulation by
arginine vasotocin and by angiotensins. In: Hazon, N., Eddy, F.B.,
Flik, G. (Eds.), Ionic Regulation in Animals. Springer, Berlin, pp.
Campbell, D.J., 1987. Circulating and tissue angiotensin systems. J.
Clin. Invest. 79, 1–6.
Cobb, C.S., Brown, J.A., 1992. Angiotensin II binding to tissues of the
rainbow trout, Oncorhynchus mykiss, studied by autoradiography.
J. Comp. Physiol B 162, 197–202.
Conlon, J.M., Yano, K., 1995. Kallikrein generates angiotensin II but
not bradykinin in the plasma of the urodele, Amphiuma tridactylum.
Comp. Biochem. Physiol. 110C, 305–311.
Fels, L.M., Kastner, S., Stolte, H., 1998. The hagfish kidney as a model
to study renal physiology and toxicology. In: Jorgensen, J.M.,
Lomholt, J.P., Weber, R.E., Malte, H. (Eds.), The Biology of
Hagfish. Chapman & Hall, London, pp. 347–363.
Hasegawa, Y., Nakajima, T., Sokabe, H., 1983. Chemical structure of
angiotensin formed with kidney renin in the Japanese eel Anguilla
japonica. Biomed. Res. 4, 417–420.
Joss, J.M.P., Itahara, Y., Watanabe, T.X., Nakajima, K., Takei, Y.,
1999. Teleost-type angiotensin is present in Australian lungfish,
Neoceratodus forsteri. Gen. Comp. Endocrinol. 114, 206–
Khosla, M.C., Nishimura, H., Hasegawa, Y., Bumpus, F.M., 1985.
Identification and synthesis of [1-asparagine, 5-valine, 9-glycine]
angiotensin I produced from plasma of American eel. Gen. Comp.
Endocrinol. 57, 223–233.
Kloas, W., Hanke, W., 1992. Localization and quantification of
angiotensin II (AII) binding sites in the kidney of Xenopus laevis—
Lack of AII receptors in the adrenal tissue. Gen. Comp. Endocrinol.
86, 173–183.
Kloas, W., Hanke, W., 1993. Receptors for atrial natriuretic factor
(ANF) in kidney and adrenal tissue of urodeles—lack of angiotensin
II (AII) receptors in these tissues. Gen. Comp. Endocrinol. 91,
Kobayashi, H., Takei, Y., 1996. The Renin–Angiotensin System.
Comparative Aspects. In: Zoophysiology, vol. 35. Springer, Berlin,
pp. 77–92.
Laurent, V., Salzet, M., 1996. Identification and properties of an
angiotensin-converting enzyme in the leech Theromyzon tessulatum.
Peptides 17, 737–745.
Nakayama, T., Nakajima, T., Sokabe, H., 1973. Comparative
studies of angiotensins. III. Structure of fowl angiotensin and
its identification by DNS-method. Chem. Pharm. Bull. 21, 2085–
Nishimura, H., 1987. Role of the renin–angiotensin system in
osmoregulation. In: Pang, P.K.T., Schreibman, M.P. (Eds.),
Vertebrate Endocrinology: Fundamentals and Biomedical Implications,
vol. 2. Academic Press, San Diego, pp. 157–187.
Nishimura, H., Ogawa, M., Sawyer, W.H., 1973. Renin–angiotensin
system in primitive bony fishes and a holocephalian. Am. J.
Physiol. 224, 950–956.
Rankin, J.C., Watanabe, T.X., Nakajima, K., Broadhead, C., Takei,
Y., 2004. Identification of angiotensin I in a cyclostome, Lampetra
fluviatilis. Zool. Sci. (in press).
Salzet, M., Stefano, G., 1997. A renin-like enzyme in the leech
Teromyzon tessulatum. Mol. Cell. Endocrinol. 131, 1–8.
Salzet, M., Deloffre, L., Bretton, C., Vieau, D., Schoofs, L., 2001. The
angiotensin system elements in invertebrates. Brain Res. Rev. 36,
Schimdt-Nielsen, K., 1997. Animal Physiology, fifth ed. Cambridge
University Press, Cambridge. pp. 302–354.
Takei, Y., 1988. Changes in blood volume after alteration of
hydromineral balance in conscious eels, Anguilla japonica. Comp.
Biochem. Physiol. 91 A, 293–297.
Takei, Y., Hasegawa, Y., 1990. Vasopressor and depressor effects of
native angiotensins and inhibition of these effects in the Japanese
quail. Gen. Comp. Endocrinol. 79, 12–22.
Takei, Y., Hasegawa, Y., Watanabe, T.X., Nakajima, K., Hazon, N.,
1993. A novel angiotensin I isolated from an elasmobranch fish. J.
Endocrinol. 139, 281–285.
Takei, Y., Itahara, Y., Butler, D.G., Watanabe, T.X., Oudit, G.Y.,
1998. Tetrapod-type angiotensin is present in a holostean fish,
Amia calva. Gen. Comp. Endocrinol. 110, 140–146.
Watanabe, T.X., Sokabe, H., Honda, I., Sakakibara, S., Nakayama,
T., Nakajima, T., 1977. Specific pressor activity and stability of
synthetic angiotensins. Jpn. J. Pharmacol. 27, 137–144.
292 Y. Takei et al. / General and Comparative Endocrinology 135 (2004) 286–292

Depositing User: Sara Taylor
Date Deposited: 20 Dec 2007
Last Modified: 21 Aug 2015 13:30


Downloads per month over past year

Repository Staff Only: item control page

View Item View Item

University of Huddersfield, Queensgate, Huddersfield, HD1 3DH Copyright and Disclaimer All rights reserved ©