Search:
Computing and Library Services - delivering an inspiring information environment

Tiliroside, a dietary glycosidic flavonoid inhibits TRAF-6/NF-kB/p38-mediated neuroinflammation in activated BV2 microglia

Velagapudi, Ravikanth, Aderogba, Mutallib A. and Olajide, Olumayokun A (2014) Tiliroside, a dietary glycosidic flavonoid inhibits TRAF-6/NF-kB/p38-mediated neuroinflammation in activated BV2 microglia. Biochimica et Biophysica Acta (BBA) - General Subjects, 1840 (12). pp. 3311-3319. ISSN 03044165

Metadata only available from this repository.

Abstract

Background
Tiliroside is a dietary glycosidic flavonoid which has shown in vivo anti-inflammatory activity. This study is aimed at evaluating the effect of tiliroside on neuroinflammation in BV2 microglia, and to identify its molecular targets of anti-neuroinflammatory action.

Methods
BV2 cells were stimulated with LPS + IFNγ in the presence or absence of tiliroside. TNFα, IL-6, nitrite and PGE2 production was determined with ELISA, Griess assay and enzyme immunoassay, respectively. iNOS, COX-2, phospho-p65, phospho-IκBα, phospho-IKKα, phospho-p38, phospho-MK2, phosopho-MKK3/6 and TRAF-6 were determined by western blot analysis. NF-κB activity was also investigated using a reporter gene assay in HEK293 cells. LPS-induced microglia ROS production was tested using the DCFDA method, while HO-1 and Nrf2 activation was determined with western blot.

Results
Tiliroside significantly suppressed TNFα, IL-6, nitrite and PGE2 production, as well as iNOS and COX-2 protein expression from LPS + IFNγ-activated BV2 microglia. Further mechanistic studies showed that tiliroside inhibited neuroinflammation by targeting important steps in the NF-κB and p38 signalling in LPS + IFNγ-activated BV2 cells. This compound also inhibited LPS-induced TRAF-6 protein expression in BV2 cells. Antioxidant activity of tiliroside in BV2 cells was demonstrated through attenuation of LPS + IFNγ-induced ROS production and activation of HO-1/Nrf2 antioxidant system.

Conclusions
Tiliroside inhibits neuroinflammation in BV2 microglia through a mechanism involving TRAF-6-mediated activation of NF-κB and p38 MAPK signalling pathways. These activities are possibly due, in part, to the antioxidant property of this compound.

General Significance
Tiliroside is a potential novel natural compound for inhibiting neuroinflammation in neurodegenerative disorders.

Item Type: Article
Contributors:
ContributionNameEmailORCID
AuthorVelagapudi, RavikanthUNSPECIFIEDUNSPECIFIED
AuthorAderogba, Mutallib A.UNSPECIFIEDUNSPECIFIED
AuthorOlajide, Olumayokun Ao.a.olajide@hud.ac.ukUNSPECIFIED
Subjects: R Medicine > RM Therapeutics. Pharmacology
Schools: School of Applied Sciences
Related URLs:
Depositing User: Olumayokun Olajide
Date Deposited: 16 Sep 2014 15:21
Last Modified: 16 Sep 2014 15:21
URI: http://eprints.hud.ac.uk/id/eprint/21555

Downloads

Downloads per month over past year

Repository Staff Only: item control page

View Item View Item

University of Huddersfield, Queensgate, Huddersfield, HD1 3DH Copyright and Disclaimer All rights reserved ©