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Molecular biological detection of anaerobic gut fungi (Neocallimastigales) from landfill sites

Lockhart, Robert J., Van Dyke, Michelle L., Beadle, Ian R., Humphreys, Paul and McCarthy, Alan J. (2006) Molecular biological detection of anaerobic gut fungi (Neocallimastigales) from landfill sites. Applied and Environmental Microbiology, 72 (8). pp. 5659-5661. ISSN 0099-2240

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Oligonucleotide primers were designed for the 18S rRNA genes of members of the Neocallimastigales and used in a nested PCR protocol to amplify 787-bp fragments of DNA from landfill site samples. The specificities of the primers were confirmed by phylogenetic analysis of the environmental clone sequences, and this method can therefore now be used to investigate the ecology of the obligately anaerobic fungi. To our knowledge, this is the first demonstration of the occurrence of members of the Neocallimastigales outside the mammalian gut, and their distribution across the landfill samples examined here suggests that they are actively involved in cellulose degradation.

Item Type: Article
Additional Information: Copyright © 2006, American Society for Microbiology.
Uncontrolled Keywords: molecular biological anaerobic gut fungi neocallimastigales landfill sites
Subjects: G Geography. Anthropology. Recreation > G Geography (General)
G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QR Microbiology
Schools: School of Applied Sciences
Depositing User: Sara Taylor
Date Deposited: 20 Dec 2007
Last Modified: 28 Jul 2010 18:20


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