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Development of A Growth Medium Suitable for Exopolysaccharide Production and Structural Characterisation by Bifidobacteria animalis ssp. lactis

Alhudhud, M, Humphreys, Paul and Laws, Andrew P. (2014) Development of A Growth Medium Suitable for Exopolysaccharide Production and Structural Characterisation by Bifidobacteria animalis ssp. lactis. Journal of Microbiological Methods, 100. pp. 93-98. ISSN 0167-7012

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Abstract

Exopolysaccharides (EPSs) produced by Bifidobacteria have received considerable attention due to their ability to modify the rheological and physicochemical properties of dairy products. However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).

This study has developed a medium based on RCM which both supports the growth of Bifidobacterium animalis ssp. lactis AD011 and does not interfere with the quantification and characterisation of the EPS generated. Medium development involved the identification of EPE-E containing components via NMR analysis followed by their removal, substitution or pre-treatment. Both beef extract and casein acid hydrolysate required chemical pre-treatment to remove polysaccharide components before the medium was free of EPS-E materials. Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources. Glucose was found to be the optimum carbon source. The final medium composition supported growth to the same extent as RCM providing significant EPS yields and no interferences during analysis

Item Type: Article
Subjects: Q Science > QR Microbiology
Schools: School of Applied Sciences > Biomolecular Sciences Research Centre
Related URLs:
Depositing User: Paul Humphreys
Date Deposited: 27 Mar 2014 15:09
Last Modified: 04 Nov 2015 16:40
URI: http://eprints.hud.ac.uk/id/eprint/19892

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