Atkinson, Gemma C., Kuzmenko, Anton, Chicherin, Ivan, Soosaar, Axel, Tenson, Tanel, Carr, Martin, Kamenski, Piotr and Hauryliuk, Vasili (2014) An evolutionary ratchet leading to loss of elongation factors in eukaryotes. BMC Evolutionary Biology, 14 (1). p. 35. ISSN 1471-2148
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Available under License Creative Commons Attribution.
The GTPase eEF1A is the eukaryotic factor responsible for the essential, universal function of aminoacyl-tRNA delivery to the ribosome. Surprisingly, eEF1A is not universally present in eukaryotes, being replaced by the paralog EFL independently in multiple lineages. The driving force behind this unusually frequent replacement is poorly understood.
Through sequence searching of genomic and EST databases, we find a striking association of eEF1A replacement by EFL and loss of eEF1A’s guanine exchange factor, eEF1Bα, suggesting that EFL is able to spontaneously recharge with GTP. Sequence conservation and homology modeling analyses indicate several sequence regions that may be responsible for EFL’s lack of requirement for eEF1Bα.
We propose that the unusual pattern of eEF1A, eEF1Bα and EFL presence and absence can be explained by a ratchet-like process: if either eEF1A or eEF1Bα diverges beyond functionality in the presence of EFL, the system is unable to return to the ancestral, eEF1A:eEFBα-driven state.
|Subjects:||Q Science > Q Science (General)|
|Schools:||School of Applied Sciences|
|Depositing User:||Cherry Edmunds|
|Date Deposited:||14 Mar 2014 16:09|
|Last Modified:||05 Dec 2016 13:47|
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