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Lactate supply as a determinant of the distribution of intracellular pH within the hepatic lobule

Burns, Shamus P., Murphy, Helena C., Iles, Richard A. and Cohen, Robert D. (2001) Lactate supply as a determinant of the distribution of intracellular pH within the hepatic lobule. Biochemical Journal, 3. pp. 569-571. ISSN 0264-6021

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When isolated livers from starved rats are perfused with lactate at constant perfusate pH and Pco2, there is a marked gradient of cell pH (pHi) along the length of the lobular radius, with periportal cells being substantially more alkaline than perivenous cells. In the present studies, the perivenous 21% of the lobular volume was destroyed by retrograde digitonin perfusion, and antegrade perfusion restored. pHi was determined by 31P-NMR. The remaining periportal cells, the site of gluconeogenesis from lactate, had a substantially higher mean pHi (7.42) than did the intact liver (7.23). When lactate was removed from the perfusate, mean pHi decreased to 7.25. The corresponding concentration of cell bicarbonate fell with a half-time of approximately 5min. When lactate was re-introduced mean pHi rose to 7.34. We conclude that a major contributor to periportal alkalinity under these conditions is proton consumption during gluconeogenesis from lactate ions.

Item Type: Article
Additional Information: © 2001 Biochemical Society
Uncontrolled Keywords: hepatic intracellular pH, hepatic intralobular heterogeneity, gluconeogenesis, digitonin
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Schools: School of Applied Sciences

1 Burns, S. P., Cohen, R. D., Iles, R. A., Germain, J. P., Going, T. C. H., Evans, S. J. W.
and Royston, P. (1996) A method for the determination in situ of variations within
the hepatic lobule of hepatocyte function and metabolite concentrations. Biochem. J.
319, 377±383
2 Burns, S. P., Murphy, H. C., Iles, R. A., Bailey, R. A. and Cohen, R. D. (2000)
Hepatic intralobular mapping of fructose metabolism in the rat liver. Biochem. J. 349,
3 Monson, J. P., Smith, J. A., Cohen, R. D. and Iles, R. A. (1982) Evidence for a lactate
transporter in the plasma membranes of rat hepatocytes. Clin. Sci. 62, 411±420
4 Fafournoux, P., De! migne! , C. and Re! me! sy, C. (1985) Carrier-mediated uptake of
lactate in rat hepatocytes. J. Biol. Chem. 260, 292±299
5 Cohen, R. D., Henderson, R. M., Iles, R. A., Monson, J. P. and Smith, J. A. (1982)
The techniques and uses of intracellular pH measurement. In ` Metabolic Acidosis ',
Ciba Foundation Symposium No. 87 (Porter, R. and Lawrenson, G., eds.), pp. 20±35,
Pitman, London
6 Cohen, R. D. (1990) The metabolic background to acid-base homeostasis and some
of its disorders. In ` The Metabolic and Molecular Basis of Acquired Disease ' (Cohen,
R. D., Lewis, B., Alberti, K. G. M. M. and Denman, A. M., eds.), pp. 962±1001,
Balliere Tindall, London
7 Burns, S. P., Desai, M., Cohen, R. D., Hales, C. N., Iles, R. A., Germain, J. P., Going,
T. C. H. and Bailey, R. A. (1997) Gluconeogenesis, glucose handling, and structural
changes in livers of the adult offspring of rats partially deprived of protein during
pregnancy and lactation. J. Clin. Invest. 100, 1768±1774
8 Burns, S. P., Cohen, R. D., Iles, R. A., Bailey, R. A., Desai, M., Germain, J. P. and
Going, T. C. D. (1999) Zonation of gluconeogenesis, ketogenesis and intracellular pH
in livers from normal and diabetic ketoacidotic rats : evidence for intralobular plasticity
of metabolic events in ketoacidosis. Biochem. J. 343, 273±280
9 Cohen, R. D, Iles, R. A, Barnett, D., Howell, M. E. O. and Strunin, J. (1971) The effect
of change in lactate uptake on the intracellular pH of the perfused rat liver. Clin. Sci.
41, 159±170
10 Cohen, R. D., Iles, R. A. and Lloyd, M. H. (1973) Perfusion of the rat liver. In
` Isolated organ perfusion ' (Ritchie, H. D. and Hardcastle, J. D., eds.), pp. 120±134,
Staples, London
11 Graf, J., Henderson, R. M., Meier, P. J. and Boyer, J. L. (1988) Regulation of
intracellular pH in hepatocytes. In `pH Homeostasis : Mechanisms and Control
(Ha$ ussinger, D., ed.), pp. 43±60, Academic Press, London
12 Fitz, J. G., Lidofsky, S. D., Xie, M-H., Cochran, M. and Scharschmidt, B. F. (1991)
Plasma membrane H+-HCO−
3 transport in rat hepatocytes : a principal role for
Na+- coupled HCO−
3 transport. Am. J. Physiol. 261, G803±G809

Depositing User: Sara Taylor
Date Deposited: 20 Dec 2007
Last Modified: 28 Jul 2010 18:20


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