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Dwelling in the dark: procedures for the crystallography of phytochromes and other photochromic proteins

Mailliet, Jo, Psakis, Georgios, Schroeder, Claudia, Kaltofen, Sabine, Dürrwang, Ulrike, Hughes, Jon and Essen, Lars-Oliver (2009) Dwelling in the dark: procedures for the crystallography of phytochromes and other photochromic proteins. Acta Crystallographica Section D: Biological Crystallography, 65 (11). pp. 1232-1235. ISSN 1399-0047

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Abstract

Crystallization of phytochromes and other photochromic proteins is hampered by the conformational changes that they undergo on exposure to light. As a canonical phytochrome, cyanobacterial Cph1 switches between two stable states upon absorption of red/far-red light. Consequently, it is mandatory to work in darkness from protein purification to crystal cryoprotection in order to ensure complete occupancy of one state or the other. With the simple and inexpensive methods that have been developed, phytochromes and other photochromic molecules can effectively be handled and crystallized, as has been demonstrated by the solution of the three-dimensional structure of the Cph1 sensory module.

Item Type: Article
Subjects: Q Science > Q Science (General)
Q Science > QC Physics
Q Science > QD Chemistry
Schools: School of Applied Sciences
Depositing User: Georgios Psakis
Date Deposited: 05 Sep 2013 10:36
Last Modified: 05 Sep 2013 10:40
URI: http://eprints.hud.ac.uk/id/eprint/18228

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