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Macromolecular conformation of chitosan in dilute solution: A new global hydrodynamic approach

Morris, Gordon, Castile, J., Smith, A., Adams, G. G. and Harding, S. E. (2009) Macromolecular conformation of chitosan in dilute solution: A new global hydrodynamic approach. Carbohydrate Polymers, 76 (4). pp. 616-621. ISSN 0144-8617

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Chitosans of different molar masses were prepared by storing freshly prepared samples for up to 6 months at either 4, 25 or 40 °C. The weight-average molar masses, Mw and intrinsic viscosities, [η] were then measured using size exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALLS) and a "rolling ball" viscometer, respectively. The solution conformation of chitosan was then estimated from:(a)the Mark-Houwink-Kuhn-Sakurada (MHKS) power law relationship [η] = kMwa and(b)the persistence length, Lp calculated from a new approach based on equivalent radii [Ortega, A., & Garcia de la Torre, J. (2007). Equivalent radii and ratios of radii from solution properties as indicators of macromolecular conformation, shape, and flexibility. Biomacromolecules, 8, 2464-2475]. Both the MHKS power law exponent (a = 0.95 ± 0.01) and the persistence length (Lp = 16 ± 2 nm) are consistent with a semi-flexible rod type (or stiff coil) conformation for all 33 chitosans studied. A semi-flexible rod conformation was further supported by the Wales-van Holde ratio, the translational frictional ratio and sedimentation conformation zoning. © 2008 Elsevier Ltd. All rights reserved.

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Item Type: Article
Uncontrolled Keywords: Chitosan Equivalent radii Intrinsic viscosity Molar mass Molar weight Sedimentation coefficient Semi-flexible rod conformation Biological membranes Chitin Chromatographic analysis Fluid dynamics Hydrogels Land use Light scattering Macromolecules Multilayers Polyethylene terephthalates Size exclusion chromatography Viscosity
Subjects: Q Science > QD Chemistry
Schools: School of Applied Sciences
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Language of Original Document: English
Correspondence Address: Morris, G.A.; National Centre for Macromolecular Hydrodynamics, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, LE12 5RD, United Kingdom; email:
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Depositing User: Gordon Morris
Date Deposited: 11 Dec 2012 12:21
Last Modified: 04 Nov 2015 21:15

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