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Alginate hydrogel has a negative impact on in vitro collagen 1 deposition by fibroblasts

Smith, Alan M., Hunt, Nicola C, Shelton, Richard M, Birdi, Gurpreet and Grover, Liam Michael (2012) Alginate hydrogel has a negative impact on in vitro collagen 1 deposition by fibroblasts. Biomacromolecules, 13 (2). pp. 4032-4038. ISSN 1525-7797

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    Abstract

    Hydrogels have been widely investigated as 3D culture substrates because of their reported structural similarity to the extracellular matrix (ECM). Limited ECM deposition, however, occurs within these materials, so the resulting “tissues” bear little resemblance to those found in the body. Here matrix deposition by fibroblasts encapsulated within a calcium alginate (Ca-alg) hydrogel was investigated. Although the cells transcribed mRNA for coll Iα over a period of 3 weeks, very little collagen protein deposition was observed within the gel by histology or immunohistochemistry (IHC). Although molecular diffusion demonstrated charge dependency, this did not prevent the flux of both positively and negative charged amino acids through the gel, suggesting that the absence of ECM could not be attributed to substrate limitation. The flux of protein, however, was charge-dependent as proteins with a net negative charge passed quickly through the Ca-alg into the medium. The minimal collagen deposition within the Ca-alg was attributed to a combination of rapid movement of negatively charged procollagen through the gel and steric hindrance of fibril formation.

    Item Type: Article
    Additional Information: Reprinted with permission from Biomacromolecules DOI: 10.1021/bm301321d Publication Date (Web): October 19, 2012 Copyright © 2012 American Chemical Societ
    Subjects: Q Science > Q Science (General)
    Q Science > QH Natural history > QH301 Biology
    R Medicine > RK Dentistry
    R Medicine > RL Dermatology
    R Medicine > RM Therapeutics. Pharmacology
    R Medicine > RS Pharmacy and materia medica
    T Technology > TA Engineering (General). Civil engineering (General)
    T Technology > TP Chemical technology
    Schools: School of Applied Sciences
    Related URLs:
    Depositing User: Alan Smith
    Date Deposited: 15 Nov 2012 16:06
    Last Modified: 19 Jun 2013 12:09
    URI: http://eprints.hud.ac.uk/id/eprint/15704

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