Search:
Computing and Library Services - delivering an inspiring information environment

Reactive intermediates in the H-phosphonate synthesis of oligonucleotides

Powles, Nicholas, Atherton, John H. and Page, Michael I. (2012) Reactive intermediates in the H-phosphonate synthesis of oligonucleotides. Organic and Biomolecular Chemistry, 10 (30). pp. 5940-5947. ISSN 1477-0539

Metadata only available from this repository.

Abstract

The formation of H-phosphonate diesters is an important step in the synthesis of oligonucleotides. Using diphenylchlorophosphate as the activator for the coupling step is often accompanied by side reactions as a result of self ‘capping’ and other reactions of the reactive intermediate. In the absence of base, the activation of ethyl H-phosphonate with diphenylchlorophosphate probably occurs through the intermediate formation of bis diethyl pyro-di-H-phosphonate rather than the expected diphenyl ethyl pyro-H-phosphonate. Pyridine acts as a nucleophilic catalyst converting diphenylchlorophosphate to its pyridinium adduct. Several side and unwanted reactions are quantified so that conditions to minimise these can be identified.

Item Type: Article
Subjects: Q Science > QD Chemistry
Schools: School of Applied Sciences
Related URLs:
Depositing User: Graham Stone
Date Deposited: 10 Oct 2012 18:10
Last Modified: 10 Oct 2012 18:10
URI: http://eprints.hud.ac.uk/id/eprint/15506

Item control for Repository Staff only:

View Item

University of Huddersfield, Queensgate, Huddersfield, HD1 3DH Copyright and Disclaimer All rights reserved ©