Single primer extension (SPEX) amplification to accurately genotype highly damaged DNA templates

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Brotherton, Paul, Endicott, Phillip, Beaumont, Mark, Barnett, Ross, Austin, Jeremy, Cooper, Alan and Sanchez, Juan J. (2008) Single primer extension (SPEX) amplification to accurately genotype highly damaged DNA templates. Forensic Science International: Genetics Supplement Series, 1 (1). pp. 19-21. ISSN 18751768

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Official URL: http://dx.doi.org/10.1016/j.fsigss.2007.10.111

DOI: 10.1016/j.fsigss.2007.10.111

Abstract

High levels of non-authentic sequence data can be generated by traditional PCR-based methodologies when DNA is damaged, template numbers are small and/or the target amplification size too large. We therefore present an alternate methodology based on single primer extension (SPEX) amplification; that places no pre-defined size constraints on amplification and interacts with only one of the DNA strands at the target locus.

Item Type:	Article
Subjects:	Q Science > QH Natural history > QH426 Genetics
Schools:	School of Applied Sciences
Depositing User:	Graham Stone
Date Deposited:	10 Oct 2012 11:36
Last Modified:	10 Oct 2012 11:36
URI:	http://eprints.hud.ac.uk/id/eprint/15472

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