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Single primer extension (SPEX) amplification to accurately genotype highly damaged DNA templates

Brotherton, Paul, Endicott, Phillip, Beaumont, Mark, Barnett, Ross, Austin, Jeremy, Cooper, Alan and Sanchez, Juan J. (2008) Single primer extension (SPEX) amplification to accurately genotype highly damaged DNA templates. Forensic Science International: Genetics Supplement Series, 1 (1). pp. 19-21. ISSN 18751768

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Abstract

High levels of non-authentic sequence data can be generated by traditional PCR-based methodologies when DNA is damaged, template numbers are small and/or the target amplification size too large. We therefore present an alternate methodology based on single primer extension (SPEX) amplification; that places no pre-defined size constraints on amplification and interacts with only one of the DNA strands at the target locus.

Item Type: Article
Subjects: Q Science > QH Natural history > QH426 Genetics
Schools: School of Applied Sciences
Depositing User: Graham Stone
Date Deposited: 10 Oct 2012 11:36
Last Modified: 10 Oct 2012 11:36
URI: http://eprints.hud.ac.uk/id/eprint/15472

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