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Global hydrodynamic analysis of the molecular flexibility of galactomannans

Morris, Gordon, Patel, T. R., Picout, D. R., Ross-Murphy, S. B., Ortega, A., Garcia de la Torre, J. and Harding, S. E. (2008) Global hydrodynamic analysis of the molecular flexibility of galactomannans. Carbohydrate Polymers, 72 (2). pp. 356-360. ISSN 0144-8617

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    Abstract

    In the past, intrinsic viscosity and sedimentation velocity analyses have been used separately to assess the conformation and flexibility of guar and locust bean gum galactomannans based on worm-like chain and semi-flexible coil models. Publication of a new global method combining data sets of both intrinsic viscosity and sedimentation coefficient with molecular weight, and minimising a target (error) function now permits a more robust analysis. Using this approach, values for the persistence length of (10 ± 2) nm for guar and (7 ± 1) nm for locust bean gum are returned if the mass per unit length ML is floated as a variable. Using a fixed mass per unit length based on the known compositional data of each galactomannan yields a similar value for Lp in both cases, (8 ± 1) nm for guar and (9 ± 1) nm for locust bean gum, with combined set of data yielding (9 ± 1) nm: within experimental error the flexibilities of both galactomannans are very similar. © 2007 Elsevier Ltd. All rights reserved.

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    Item Type: Article
    Uncontrolled Keywords: Galactomannan Guar LBG Mass per unit length Persistence length Semi-flexible coil Worm-like chain Data structures Hydrodynamics Mathematical models Robust control Viscosity Galactomannans Intrinsic viscosity Molecular flexibility Sedimentation Cyamopsis tetragonoloba
    Subjects: Q Science > QD Chemistry
    Schools: School of Applied Sciences
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    Language of Original Document: English
    Correspondence Address: Morris, G.A.; National Centre for Macromolecular Hydrodynamics, School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough, LE12 5RD, United Kingdom; email: gordon.morris@nottingham.ac.uk
    References: Bohdanecky, M., New method for estimating the parameters of the wormlike chain model from the intrinsic viscosity of stiff-chain polymers (1983) Macromolecules, 16, pp. 1483-1492; Bohdanecky, M., Petrus, V., Analysis of hydrodynamic data for denatured globular proteins in terms of the worm like cylinder model (1991) International Journal of Biological Macromolecules, 13, pp. 231-234; Clark, A.H., Dea, I.C.M., McCleary, B.V., (1985) The effect of the galactomannan fine structure on their interaction properties (3rd ed.), , Elsevier Applied Science Publishers, London pp 429-465; Dea, I.C.M., Structure/function relationships of galactomannans and food grade cellulosics (1990) Gums and Stabilisers for the Food Industry, 5, pp. 373-382. , Phillips G.O., Wedlock D.J., and Williams P.A. (Eds), IRL Press, Oxford; Harding, S.E., The intrinsic viscosity of biological macromolecules. Progress in measurement, interpretation and application to structure in dilute solution. (1997) Progress in Biophysics and Molecular Biology, 68, pp. 207-262; Hearst, J.E., Rotatory diffusion constants of stiff-chain macromolecules (1963) Journal of Chemical Physics, 38, pp. 1062-1065; Hearst, J.E., Stockmayer, W.H., Sedimentation constants of broken chains and wormlike coils (1962) Journal of Chemical Physics, 37, pp. 1425-1433; Kratky, O., Porod, G., Röntgenungtersuchung gelöster Fadenmoleküle (1949) Recueil Des Travaux Chimiques Des Pays-Bas, 68, pp. 1106-1109; Ortega, A., & García de la Torre, J. (2007). Equivalent radii and ratios of radii from solution properties as indicators of macromolecular conformation, shape and flexibility. Biomacromolecules (accepted) 10.1021/bm700473f available on-line 27/07/2007. See also Ortega, A. (2005). Metodologías computacionales para propiedades en disolución de macromoléculas rígidas y flexibles. PhD Dissertation, Universidad de MurciaPatel, T.R., Picout, D.R., Ross-Murphy, S.B., Harding, S.E., Pressure cell assisted solution characterisation of polysaccharides. 3. Application of analytical ultracentrifugation techniques (2006) Biomacromolecules, 7, pp. 3513-3520; Petkowitz, C.L.O., Reicher, F., Mazeau, K., Conformation analysis of galactomannans: From oligomeric segments to polymeric chains (1998) Carbohydrate Polymers, 37, pp. 25-39; Picout, D.R., Ross-Murphy, S.B., Errington, N., Harding, S.E., Pressure cell assisted solution characterisation of polysaccharides. 1. Guar gum (2001) Biomacromolecules, 2, pp. 1301-1309; Picout, D.R., Ross-Murphy, S.B., Jumel, K., Harding, S.E., Pressure cell assisted solution characterisation of polysaccharides. 2. Locust bean gum (2002) Biomacromolecules, 3, pp. 761-767; Stockmayer, W.H., Fixman, M.J., On the estimation of unperturbed dimensions from intrinsic viscosities (1963) Journal of Polymer Science C, 1, pp. 137-141; Tanford, C., (1961) Physical chemistry of macromolecules, , John Wiley & Sons, New York pp. 343-344; Tombs, M.P., Harding, S.E., (1998) An introduction to polysaccharide biotechnology, , Taylor and Francis, London pp. 14-20; Yamakawa, H., Fujii, M., Translational friction coefficient of wormlike chains (1973) Macromolecules, 6, pp. 407-415

    Depositing User: Gordon Morris
    Date Deposited: 18 Sep 2012 14:32
    Last Modified: 18 Sep 2012 14:32
    URI: http://eprints.hud.ac.uk/id/eprint/14613

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