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Investigation into the physical and chemical properties of sodium caseinate-maltodextrin glyco-conjugates

Morris, Gordon, Sims, I. M., Robertson, A. J. and Furneaux, R. H. (2004) Investigation into the physical and chemical properties of sodium caseinate-maltodextrin glyco-conjugates. Food Hydrocolloids, 18 (6). pp. 1007-1014. ISSN 0268-005X

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    Abstract

    Casein-maltodextrin glyco-conjugates were prepared using an economical, food-grade process based on the Amadori re-arrangement of the Maillard reaction. The resultant glyco-conjugates were slightly yellow in colour and the degree of discolouration was dependent on heating time. Formation of glyco-conjugates was demonstrated by determining the reduction of free amino-groups by the O-phthaldialdehyde (OPA) assay and sugar reducing ends by gas chromatography-mass spectrometry (GC-MS). Increases in molecular weight were monitored by SDS-polyacrylamide gel electrophoresis (PAGE) and were in agreement with those predicted for the conjugation of casein monomers with malto-oligosaccharides of average DP 7-10. 2D-Urea-SDS-PAGE demonstrated that both protein and saccharide components co-migrate, indicating that covalent bonds were upon heating. This resulted in increases in mass-to-charge ratio of the materials, which suggested decreases in pI. These observed chemical and physical changes were reconciled with previously documented improvements in emulsifying properties. © 2004 Elsevier Ltd. All rights reserved.

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    Item Type: Article
    Additional Information: NOTICE: this is the author’s version of a work that was accepted for publication in Food Hydrocolloids Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Food Hydrocolloids Volume 18, Issue 6, November 2004, Pages 1007–1014
    Uncontrolled Keywords: Amadori Available lysine Caseinate Gas chromatography-mass spectrometry Glyco-conjugate Maltodextrin Reducing ends SDS-polyacrylamide gel electrophoresis carbohydrate casein monomer oligosaccharide phthalaldehyde protein sodium derivative sugar unclassified drug urea Amadori rearrangement article assay chemical analysis color conjugate conjugation covalent bond economic aspect emulsion food processing gas chromatography glycation heating mass mass spectrometry materials molecular weight monitoring physical chemistry polyacrylamide gel electrophoresis prediction protein analysis reaction analysis reduction time
    Subjects: Q Science > QD Chemistry
    Schools: School of Applied Sciences
    Related URLs:
    References:

    Language of Original Document: English
    Correspondence Address: Morris, G.A.; Industrial Research Ltd., PO Box 31-310, Gracefield Research Centre, Lower Hutt, New Zealand; email: morris@nantes.inra.fr
    Chemicals/CAS: casein, 9000-71-9; caseinate, 9005-46-3; maltodextrin, 9050-36-6; phthalaldehyde, 643-79-8; protein, 67254-75-5; urea, 57-13-6
    References: Akhtar, M., Dickinson, E., Emulsifying Properties of Whey Protein-Dextran Conjugates at Low pH and Different Salt Concentrations (2003) Colloids and Biosurfaces B: Biointerfaces, 31, pp. 125-132; Bertrand-Harb, C., Nicolas, M.G., Dalgalarrondo, M., Chobert, J.-M., Determination of the alkylation degree by 3 colorimetric methods and amino-acid-analysis - A comparative-study (1993) Sciences des Aliments, 13, pp. 577-584; Carlsson, S., Isolation and characterisation of glycoproteins (1993) Glycobiology - A Practical Approach, , M. Fukuda, & A. Kobata. Oxford: IRL Press; Chevalier, F., Chobert, J.-M., Popineau, Y., Nicolas, M.G., Haertlé, T., Improvement of functional properties of b-lactoglobulin glycated through maillard reaction is related to the nature of the sugar (2001) International Dairy Journal, 11, pp. 145-152; Davies, D.T., Law, A.J.R., An improved method for the quantitative fractionation of casein mixtures using ion-exchange chromatography (1977) Journal of Dairy Research, 44, pp. 213-221; Dickinson, E., Towards more natural emulsifiers (1993) Trends in Food Science and Technology, 4, pp. 330-334; Dinnella, C., Gargaro, M.T., Rossano, R., Monteleone, E., Spectrophotometric assay using o-phthaldialdehyde for the determination of transglutaminase on casein (2002) Food Chemistry, 78, pp. 363-368; Fayle, S.E., Gerrard, J.A., (2002) The Maillard Reaction, , Cambridge, UK: Royal Society of Chemistry; Fayle, S.E., Healy, J.P., Brown, P.A., Reid, E.A., Gerrard, J.A., Ames, J.M., Novel approaches to the analysis of Maillard reaction proteins (2001) Electrophoresis, 22, pp. 1518-1525; Gerrard, J.A., Fayle, S.E., Sutton, K.H., Covalent protein adduct formation and protein cross-linking resulting from the Maillard reaction between cyclotene and a model food protein (1999) Journal of Agriculture and Food Chemistry, 47, pp. 1183-1188; Handa, A., Kuroda, N., Functional Improvement in Dried Egg White through the Maillard Reaction (1999) Journal of Agriculture and Food Chemistry, 47, pp. 1845-1850; Hattori, M., Numamoto, K.-I., Kobayashi, K., Takahashi, K., Functional changes in β-lactoglobulin by conjugation with cationic saccharides (2000) Journal of Agriculture and Food Chemistry, 48, pp. 2050-2056; Hattori, M., Okada, Y., Okada, Takahashi, K., Functional changes in β-lactoglobulin upon conjugation with carboxymethyl cyclodextrin (2000) Journal of Agriculture and Food Chemistry, 48, pp. 3789-3794; Kato, A., Functional protein-polysaccharide conjugates (1996) Comments on Agriculture and Food Chemistry, 3, pp. 139-153; Kato, Y., Matsuda, T., Kato, N., Nakamura, R., Browning and protein polymerization induced by amino-carbonyl reaction of ovalbumin with glucose and lactose (1988) Journal of Agriculture and Food Chemistry, 36, pp. 806-809; Kato, A., Mifuru, R., Matsudomi, N., Kobayashi, K., Functional casein-polysaccharide conjugates prepared by controlled dry heating (1992) Bioscience, Biotechnology and Biochemistry, 56, pp. 567-571; Kato, A., Minoki, K., Kobayashi, K., Improvement of emulsifying properties of egg white proteins by the attachment of polysaccharide through maillard reaction in a dry state (1993) Journal of Agriculture and Food Chemistry, 41, pp. 540-543; Kato, A., Sato, T., Kobayashi, K., Emulsifying properties of protein-polysaccharide complexes and hybrids (1989) Agriculture and Biological Chemistry, 53, pp. 2147-2152; Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685; Matsuda, T., Kato, A., Nakamura, R., Lysine loss and polymerization of bovine β-lactoglobulin by amino carbonyl reaction with lactulose (4-O-β-galactopyranosyl-D-fructose) (1991) Journal of Agriculture and Food Chemistry, 39, pp. 1201-1204; Mislovicová, D., Masárová, J., Ŝvitel, J., Mendichi, R., Ŝoltés, L., Gemeiner, P., Danielsson, B., Neoglycoconjugates of mannan with bovine serum albumin and their interaction with lectin concanavalin a (2002) Bioconjugate Journal, 13, pp. 136-142; Morris, G.A., The self-assembly and structure of caseins in solution (2002) Biotechnology and Genetic Engineering Reviews, 19, pp. 357-376. , Harding S.E. Wimborne, UK: Technical and Medical Publishers; Nagasawa, K., Ohgata, K., Takahashi, K., Hattori, M., Role of the polysaccharide content and net charge on the emulsifying properties of β-Lactoglobulin-carboxymethyl dextran conjugates (1996) Journal of Agriculture and Food Chemistry, 44, pp. 2538-2543; Nakamura, S., Kobayashi, K., Kato, A., Role of positive charge of lysozyme in the excellent emulsifying properties of maillard-type lysozyme-polysaccharide conjugate (1994) Journal of Agriculture and Food Chemistry, 42, pp. 2688-2691; O'Connell, J.E., Fox, P.F., Heat-induced changes in the calcium sensitivity of caseins (1999) International Dairy Journal, 9, pp. 839-847; Rufian-Henares, J.A., Guerra-Hernandez, E., Garcia-Villanova, B., Maillard reaction in enteral formula processing: Furosine, loss of O-phthaldialdehyde reactivity, and fluorescence (2002) Food Research International, 35, pp. 527-533; Semenova, M.G., Belyakova, L.E., Antipova, A.S., Jubanova, M.A., Influence of maltodextrins with different dextrose equivalents on the thermodynamic properties of legumin in a bulk and at the air-water interface (1999) Colloids and Biosurfaces B: Biointerfaces, 12, pp. 287-297; Shepherd, R., Robertson, A., Ofman, D., Dairy glycoconjugate emulsifiers: Casein-maltodextrins (2000) Food Hydrocolloids, 14, pp. 281-286; Sims, I.M., Thomson, A., Hubl, U., Larsen, N.G., Furneaux, R.H., Characterisation of polysaccharides synthesised by gluconobacter oxydans NCIMB 4943 (2001) Carbohydrate Polymers, 45, pp. 285-292; Smales, C.M., Pepper, D.S., James, D.C., Protein modification during anti-viral heat-treatment bioprocessing of factor VIII concentrates, Factor IX Concentrates, and Model Proteins in the Presence of Sucrose (2002) Biotechnology and Bioengineering, 77, pp. 37-48; Song, Y., Babiker, E.-F.E., Usui, M., Saito, A., Kato, A., Emulsifying properties and bactericidal action of chitosan-lysozyme conjugates (2002) Food Research International, 35, pp. 459-466

    Depositing User: Gordon Morris
    Date Deposited: 13 Sep 2012 16:40
    Last Modified: 13 Sep 2012 16:40
    URI: http://eprints.hud.ac.uk/id/eprint/14608

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