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Physical characterisation of the rhamnogalacturonan and homogalacturonan fractions of sugar beet (Beta vulgaris) pectin

Morris, Gordon, Ralet, M.C., Bonnin, E., Thibault, J.F. and Harding, S. E. (2010) Physical characterisation of the rhamnogalacturonan and homogalacturonan fractions of sugar beet (Beta vulgaris) pectin. Carbohydrate Polymers, 82 (4). pp. 1161-1167. ISSN 0144-8617

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    Abstract

    Acid extracted sugar beet (Beta vulgaris) pectin was subjected to enzymatic hydrolysis using fungal pectin methyl esterase (f-PME) and two endopolygalacturonanases (PGs I and II). From the hydrolysate, the RG-I fraction was separated and purified by chromatographic techniques. This RG-I fraction was shown to be of high weight average molar mass (188,000 g/mol), but low intrinsic viscosity (36 ml/g), which is consistent with a random coil conformation (Lp = 1.4 nm). The HG fraction was prepared by mild acid hydrolysis of acid extracted pectin. The HG fraction was found to have a relatively low weight average molar mass (20,000 g/mol), but a rather high intrinsic viscosity (77 ml/g), which is consistent with the HG fraction being rigid in solution (Lp = 9.8 nm). Lower molar mass pectins are richer in HG regions and pectins of higher molar mass are richer in RG-I regions. We conclude that the degradation of the HG region has an important impact on intrinsic viscosity, but less on molar mass and the inverse is true for the degradation of RG-I region. This has important consequences in terms of the functionality of sugar beet pectin molecules. © 2010 Elsevier Ltd.

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    Item Type: Article
    Additional Information: NOTICE: this is the author’s version of a work that was accepted for publication in Carbohydrate Polymers Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Carbohydrate Polymers Volume 82, Issue 4, 11 November 2010, Pages 1161–1167
    Uncontrolled Keywords: Acid hydrolysis Endopolygalacturonase (endo-Pg) Homogalacturonan (Hg) region Rhamnogalacturonan (RG-I) region Sugar beet (Beta vulgaris) pectin Average molar mass Beta vulgaris Chromatographic techniques Endopolygalacturonase Homogalacturonan Intrinsic viscosity Low weight Mild acid hydrolysis Pectin methyl esterase Physical characterisation Random coil conformation Rhamnogalacturonan Acids Degradation Mercury (metal) Sugar (sucrose) Sugar beets Viscosity Enzymatic hydrolysis Beta vulgaris subsp. vulgaris
    Subjects: Q Science > QD Chemistry
    Schools: School of Applied Sciences
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    Language of Original Document: English
    Correspondence Address: Morris, G. A.; National Centre for Macromolecular Hydrodynamics (NCMH), School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom; email: gordon.morris@nottingham.ac.uk
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    Depositing User: Gordon Morris
    Date Deposited: 13 Sep 2012 16:37
    Last Modified: 13 Sep 2012 16:37
    URI: http://eprints.hud.ac.uk/id/eprint/14607

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