Solutionproperties of capsularpolysaccharides from Streptococcus pneumoniae

Harding, Stephen E., Abdelhameed, A. S., Morris, Gordon, Adams, G. G., Laloux, O., Cerny, L., Bonnier, B., Duvivier, P., Conrath, K. and Lenfant, C. (2012) Solutionproperties of capsularpolysaccharides from Streptococcus pneumoniae. Carbohydrate Polymers, 90 (1). pp. 237-242. ISSN 0144-8617

Abstract

Capsular polysaccharides from ten different serotypes of Streptococcus pneumoniae have been stud-ied with regards their hydrodynamic properties in solution, namely their sedimentation coefficient and molar mass distributions, solution conformations and flexibilities (persistence lengths L p), impor-tant properties for the construction of polysaccharide and glycoconjugate vaccines. Sedimentation and molar mass distributions (obtained by sedimentation velocity and equilibrium analysis in the analytical ultracentrifuge supported by size exclusion chromatography coupled to multi-angle light scattering mea-surements) were generally unimodal, with weight (mass) average molar masses ranging from 100 × 10 3 to 1300 × 10 3 g/mol. Estimates of chain flexibilities from three different procedures applied to intrinsic viscosity, sedimentation coefficient and molar mass data, showed that the polysaccharides from all the serotypes studied had semi-flexible structures in solution with persistence lengths in the range from ∼4 to 9 nm.

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