Search:
Computing and Library Services - delivering an inspiring information environment

Identification of Angiotensin I in a Cyclostome, Lampetra fluviatilis

Rankin, J. Cliff, Watanabe, Takushi X., Nakajima, Kiichiro, Broadhead, Caren and Takei, Yoshio (2004) Identification of Angiotensin I in a Cyclostome, Lampetra fluviatilis. Zoological science, 21 (2). pp. 173-179. ISSN 0289-0003

Metadata only available from this repository.

Abstract

Angiotensin I (ANG I) was isolated from incubates of plasma and kidney extracts of the river lamprey, Lampetra fluviatilis, using eel vasopressor activity as an assay during purification. Its sequence was Asn-Arg-Val-Tyr-Val-His-Pro-Phe-Thr-Leu as determined by the sequence analysis and mass spectrometry. The sequence was confirmed by identity of the elution profile with the synthetic peptide in two different reverse-phase columns of high-performance liquid chromatography. Lamprey ANG I produced dorsal-aortic pressor responses in L. fluviatilis but the rise was very small in comparison to that produced by angiotensin II. Angiotensin III produced an even bigger increase. It was not possible to demonstrate a difference in response to Asn1 (lamprey) ANG I and Asp1 (human) ANG I. The present study directly demonstrated the presence and biological activity of the renin-angiotensin system in the most primitive extant vertebrates, the cyclostomes. Thus the renin-angiotensin system is a phylogenetically old hormonal system that is present throughout the vertebrates.

Item Type: Article
Subjects: Q Science > Q Science (General)
Q Science > QL Zoology
Schools: School of Applied Sciences
Related URLs:
Depositing User: Sara Taylor
Date Deposited: 11 Aug 2008 11:35
Last Modified: 11 Aug 2008 11:35
URI: http://eprints.hud.ac.uk/id/eprint/1447

Item control for Repository Staff only:

View Item

University of Huddersfield, Queensgate, Huddersfield, HD1 3DH Copyright and Disclaimer All rights reserved ©