Single Channel Simultaneous Analysis of DNA and MicroRNA

Williams, Graham (2012) Single Channel Simultaneous Analysis of DNA and MicroRNA. In: Proceedings of the American Academy of Forensic Sciences. 2012 AAFS Annual Meeting, 18 . American Academy of Forensic Sciences AAFS, Atlanta, Georgia, USA, p. 34.

Abstract

After attending this presentation, attendees will be aware of a new
proposed protocol that will analyse DNA and miRNA simultaneously.
This presentation will impact the forensic science community by
exhibiting a new protocol that will expand the capability of forensic science
laboratories with minimal modifications and expenses.
It is often necessary to establish the identity of the body fluid from
which a DNA profile was obtained. This issue is frequently encountered in
sexual offences where it can be necessary to distinguish between saliva and
vaginal material or where there is a very small amount of the body fluid
present, such as trace amounts of blood on a dark surface. This issue is
currently being resolved by the use of mRNA and microRNA (miRNA)
analysis utilising a co-isolation technique to separate out the DNA and the
miRNA phases for subsequent analysis.

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