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Molecular Modelling of the Complex Polysaccharide Heparan Sulphate

Swift, Simone (2011) Molecular Modelling of the Complex Polysaccharide Heparan Sulphate. Doctoral thesis, University of Huddersfield.

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    Abstract

    Heparan Sulphate plays an important role in many life processes and so an understanding of its
    role as a universal co-receptor is of great importance. Traditionally oligosaccharides derived from the related molecule heparin have been at the forefront of molecular drug design, due to its similarity in structure and function. To obtain a more complex and detailed picture of the role of HS in structural biology further complex work must be undertaken on HS. Here in this study a number of HS derived octasaccharides have been purified. Alongside this is work carried on HS derived decasaccharides, of which all have undergone extensive molecular modelling
    simulations. The role of the Iduronates in the HS structure, at monosaccharide level has indicated
    a major role for these structures in biological activity. Further work has indicated the level of
    sulphation is also a requirement which in turn influences conformational behaviour. Up until now
    this has only really been studied at the monosaccharide level and so this study has generated a number of different HS models which can confirm the importance of iduronate conformation in biological activity. Not only that but it has also been identified that certain torsional geometries within the glycosidic bonds between monosaccharides also has a major influence on conformation. Local deviations in the molecular modelling data suggest there is a slight difference between active and inactive oligosaccharides with the ability to bind and activate the HS:FGF2:FGFR IIIc complex.

    The implications of these and other structural insights are discussed with the implication heavily towards a combination of both torsional geometry and iduronate conformation in biological
    activity. To further aid our understanding HS dp10 oligosaccharides were docked into a FGF2
    which was in a complex with a heparin hexasaccharide. Docking experiments were carried in order to attain structural information on the binding of these molecules. The Goodger
    oligosaccharides were docked into the X-ray crystal structure and were chosen specifically for
    their iduronate conformation or the torsional geometry.

    Item Type: Thesis (Doctoral)
    Subjects: Q Science > Q Science (General)
    Q Science > QD Chemistry
    Schools: School of Applied Sciences
    Depositing User: Lauren Hollingworth
    Date Deposited: 14 Jun 2011 14:50
    Last Modified: 12 May 2013 01:38
    URI: http://eprints.hud.ac.uk/id/eprint/10739

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