Molecular weight distribution evaluation of polysaccharides and glycoconjugates using analytical ultracentrifugation

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Harding, S. E., Abdelhameed, A. S. and Morris, Gordon (2010) Molecular weight distribution evaluation of polysaccharides and glycoconjugates using analytical ultracentrifugation. Macromolecular Bioscience, 10 (7). pp. 714-720. ISSN 1616-5187

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Official URL: http://dx.doi.org/10.1002/mabi.201000072

DOI: 10.1002/mabi.201000072

Abstract

We review here the advances that have been made in methodology using the analytical ultracentrifuge for characterising polydisperse polymer systems with a quasi-continuous distribution of molecular weight. These advances include improved ways of defining the weight and z-average molecular weights of a distribution from sedimentation equilibrium experiments and hence the ratio Mz=Mw, off-line calibration of size exclusion chromatography by sedimentation equilibrium and conversion of a sedimentation coefficient distribution into a molecular weight distribution. Although these methods are applicable to other polymeric systems, we focus on polysaccharides, mucins and other glycoconjugates, systems which particularly benefit from the availability of recently available long optical path length cells for the minimisation of complications through thermodynamic non-ideality. Â© 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

Item Type:	Article
Uncontrolled Keywords:	Biopolymers Chromatography Molar mass distribution Molecular weight distribution Polysaccharides Ultracentrifugation Analytical ultracentrifugation Analytical ultracentrifuge Average molecular weight Continuous distribution Glycoconjugates Long optical path Nonideality Polydisperse polymers Polymeric systems Sedimentation coefficients Sedimentation equilibrium Biomolecules Centrifugation Polydispersity Polymers Weighing Size exclusion chromatography glycoconjugate polysaccharide calibration carbohydrate analysis molecular weight review sedimentation thermodynamics Chromatography, Gel
Subjects:	Q Science > QD Chemistry
Schools:	School of Applied Sciences
Related URLs:	Author
References:	Language of Original Document: English Correspondence Address: Harding, S. E.; National Centre for Macromolecular Hydrodynamics, University of Nottingham, Sutton Bonington, LE12 5RD, United Kingdom; email: steve.harding@nottingham.ac.uk Chemicals/CAS: Glycoconjugates; Polysaccharides References: Ranby, B., (1987) Physical Chemistry of Colloids and Macromolecules: The Svedberg Symposium, , Blackwell Scientific, Oxford; Creeth, J.M., Pain, R.H., (1967) Prog. Biophys. Mol. Biol., 17, p. 217; Harding, S.E., (1984) Biochem. J., 219, p. 1061; Hall, D.R., Harding, S.E., Winzor, D.J., (1999) Prog. Coll. Polym. Sci., 113, p. 62; Yphantis, D.A., (1964) Biochemistry, 3, p. 297; Chervenka, C.H., (1970) Analyt. Biochem., 34, p. 24; Teller, D.C., (1973) Methods Enzymol., 27, p. 346; Creeth, J.M., Harding, S.E., (1982) J. Biochem. Biophys.Meth., 7, p. 25; Creeth, J.M., (1980) Biochem. Soc. Trans., 8, p. 520; Harding, S.E., Horton, J.C., Morgan, P.J., (1992) Analytical Ultracentrifugation in Biochemistry and Polymer Science, pp. 275-294. , S. E. Harding, A. J. Rowe, J. C. Horton, Eds., Royal Society of Chemistry, Cambridge; Harding, S.E., (1992) Analytical Ultracentrifugation in Biochemistry and Polymer Science, pp. 495-516. , S. E. Harding, A. J. Rowe, J. C. Horton, Eds., Royal Society of Chemistry, Cambridge; CÃ¶lfen, H., Harding, S.E., (1997) Eur. Biophys. J., 25, p. 333; http://www.nanolytics.de/Harding, S.E., Creeth, J.M., (1982) IRCS Med. Sci., 10, p. 474; Harding, S.E., (1985) Biophys. J., 47, p. 247; Rowe, A.J., Wynne-Jones, S., Thomas, D.G., Harding, S.E., (1992) Analytical Ultracentrifugation in Biochemistry and Polymer Science, pp. 49-62. , S. E. Harding, A. J. Rowe, J. C. Horton, Eds., Royal Society of Chemistry, Cambridge; http://www.nottingham.ac.uk/ncmh/unit/method.html#-SoftwareHarding, S.E., VÃ¥rum, K.M., Stokke, B.T., SmidsrÃ¸d, O., (1991) Advances in Carbohydrate Analysis, pp. 63-144. , C. A. White, Ed., JAI Press, Greenwich; Horton, J.C., Harding, S.E., Mitchell, J.R., Morton-Holmes, D.F., (1991) Food Hydrocoll., 5, p. 125; Fujita, H., (1962) Mathematical Theory of Sedimentation Analysis, , Academic Press, New York; Herdan, G., (1949) Nature, 163, p. 139; Ball, A., (1989) Molecular, Weights of Industrial Polysaccharides, , PhD Thesis, University of Nottingham; Ball, A., Harding, S.E., Mitchell, J.R., (1988) Int. J. Biol. Macromol., 10, p. 259; Ball, A., Harding, S.E., Simpkin, N.J., (1990) Gums and Stabilisers for the Food Industry, 5, p. 447; Harding, S.E., Berth, G., Ball, A., Mitchell, J.R., GarcÃ¬a De La Torre, J., (1991) Carbohyd. Polym., 16, p. 1; Cantow, H.J., (1959) Makromol. Chem., 30, p. 169; SmidsrÃ¸d, O., Andresen, L., (1979) Biopolymerkjemi, , Tapir Press, Trondheim; Harding, S.E., (1989) Adv. Carbohydr. Chem. Biochem., 47, p. 345; Pain, R.H., (1980) Symp. Soc. Exp. Biol., 34, p. 359; Sheehan, J.K., Oates, K., Carlstedt, I., (1986) Biochem. J., 239, p. 147; Schuck, P., (2005) Analytical Ultracentrifugation Techniques and Methods, pp. 26-50. , D. J. Scott, S. E. Harding, A. J. Rowe, Eds., Royal Society of Chemistry, Cambridge
Depositing User:	Gordon Morris
Date Deposited:	19 May 2011 11:47
Last Modified:	01 Jul 2011 15:02
URI:	http://eprints.hud.ac.uk/id/eprint/10465

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