Bioactive arabinogalactans from the leaves of Opilia celtidifolia Endl. ex Walp. (Opiliaceae)

Tools

Gronhaug, T. E., Ghildyal, P., Barsett, H., Michaelsen, T. E., Morris, Gordon, Diallo, D., Inngjerdingen, M. and Paulsen, B. S. (2010) Bioactive arabinogalactans from the leaves of Opilia celtidifolia Endl. ex Walp. (Opiliaceae). Glycobiology, 20 (12). pp. 1654-1664. ISSN 0959-6658

Metadata only available from this repository.

Official URL: http://dx.doi.org/10.1093/glycob/cwq120

DOI: 10.1093/glycob/cwq120

Abstract

The leaves of the tree Opilia celtidifolia have a long tradition for being used in Mali and other West African countries against various ailments such as for wound healing. Previous studies on polysaccharides from these leaves showed the presence of pectic-like polymers with an effect on the human complement system as well as the ability to activate macrophages. The present study shows that bioactive arabinogalactans isolated by water of 50Â°C could be separated into two acidic fractions, Oc50A1 and Oc50A2. The former could, by gel filtration on Sephacryl S-400, be separated into two fractions, which were further purified on a Superdex 200 column to give the fractions Oc50A1.I.pur and Oc50A1.II.pur. These fractions were subjected to chemical and biological studies. The polysaccharides consisted mainly of heavily branched type II arabinogalactans and minor amounts of rhamnogalacturonan I regions. The isolated polymers had a high human complement-fixating ability, as well as the ability to stimulate rat macrophages and dendritic cells (DCs) and to induce B cell proliferation. These effects were especially pronounced for the higher molecular weight fraction of Oc50A1.I.pur. The fractions Oc50A1.I.pur and Oc501.II.pur stimulated secretion of pro-inflammatory cytokines from purified B cells or DCs. Collectively, these results indicate that the arabinogalactan type II polymers present in the leaves of O. celtidifolia may be used to develop medical devices for regulating inflammatory processes. Â© 2010 The Author.

Item Type:	Article
Uncontrolled Keywords:	B cells cytokines dendritic cells immunomodulating activity Opilia celtidifolia Endl. ex Walp. arabinogalactan bacterium lipopolysaccharide carbohydrate cytokine monosaccharide nitric oxide polymer polysaccharide animal cell article B lymphocyte complement fixation controlled study cytokine release dendritic cell flow cytometry immunomodulation lymphocyte proliferation macrophage Mali medicinal plant molecular weight nonhuman Opilia celtidifoliai opiliaceae plant leaf priority journal protein content rat spleen cell traditional medicine tree Animals B-Lymphocytes Cell Proliferation Complement Activation Complement Fixation Tests Complement System Proteins Galactans Humans Inflammation Macrophage Activation Macrophages Plant Extracts Plant Leaves Rats Trees Wound Healing Opilia celtidifolia Rattus
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Schools:	School of Applied Sciences
Related URLs:	Author
References:	Language of Original Document: English Correspondence Address: GrÃ¸nhaug, T. E.; School of Pharmacy, Faculty of Mathematics and Natural SciencesUnited Kingdom; email: t.e.gronhaug@farmasi.uio.no Chemicals/CAS: arabinogalactan, 9036-66-2; nitric oxide, 10102-43-9; Complement System Proteins, 9007-36-7; Galactans; Plant Extracts; arabinogalactan, 9036-66-2 References: Barsett, H., Smestad Paulsen, B., Separation, isolation and characterization of acidic polysaccharides from the inner bark of Ulmus glabra Huds (1991) Carbohydr Polym, 17, pp. 137-144; Burkill, H.M., (1997) The Useful Plants of West Tropical Africa, 4. , Families M-R. Kew: Royal Botanic Gardens; Chambers, R.E., Clamp, J.R., An Assessment of methanolysis and other factors used in the analysis of carbohydrate-containing materials (1971) Biochem J, 125, pp. 1009-1018; Cipriani, T.R., Mellinger, C.G., De Souza, L.M., Baggio, C.H., Freitas, C.S., Marques, M.C.A., Paj, G., Iacomini, M., A polysaccharide from a tea (infusion) of Maytenus ilicifolia leaves with anti-ulcer protective effects (2006) J Nat Prod, 69, pp. 1018-1021; Ciucanu, I., Kerek, F., A simple and rapid method for the permethylation of carbohydrates (1984) Carbohydr Res, 131, pp. 209-217; Crespin, F., Olliver, E., Lavaud, C., Babadjamian, A., Faure, R., Debrauwer, L., Balansard, G., Boudon, G., Triterpenoid saponins from Opilia celti-difolia (1993) Phytochemistry, 33, pp. 657-661; Diallo, D., Paulsen, B.S., Tha, L., Michaelsen, T.E., Polysaccharides from the roots of Entada africana Guill. et Perr., Mimosaceae, with complement fixing activity (2001) J Ethnopharmacol, 74, pp. 159-171; Diallo, D., Sogn, C., Samake, F.B., Paulsen, B.S., Michaelsen, T.E., Keita, A., Wound healing plants in Mali, the Bamako region. An ethnobotanical survey and complement fixation of water extracts from selected plants (2002) Pharm Biol, 40, pp. 117-128; Druet, D., Comeau, L.C., Estienne, J., Isolation of an aglycone from Opilia celtidifolia: 3beta-acetoxyolean-12- ene-23alpha,28beta-dioic acid (1991) Planta Med, 57, p. 200; Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., Colorimetric method for determination of sugars and related substances (1956) Anal Chem, 28, pp. 350-356; GrÃ¸nhaug, T.E., GlÃ¦serud, S., Skogsrud, M., Ballo, N., Bah, S., Diallo, D., Paulsen, B.S., Ethnopharmacological survey of six medicinal plants from Mali, West-Africa (2008) J Ethnobiol Ethnomed, 4, p. 26; Harding, S.E., (2000) Polysaccharides/centrifugation, pp. 3921-3929. , Wilson I and Poole C, editors. Encylopedia of Separation Science III. New York: Academic Press; Harding, S.E., Challenges for the modern analytical ultracentrifuge analysis of polysaccharides (2005) Carbohydr Res, 340, pp. 811-826; Inngjerdingen, K., Nergard, C.S., Diallo, D., Mounkoro, P.P., Paulsen, B.S., An ethnopharmacological survey of plants used for wound healing in Dogon-land, Mali, West Africa (2004) J Ethnopharmacol, 92, pp. 233-244; Inngjerdingen, K.T., Debes, S.C., Inngjerdingen, M., Hokputsa, S., Harding, S.E., Rolstad, B., Michaelsen, T.E., Paulsen, B.S., Bioactive pectic polysaccharides from Glinus oppositifolius (L.) Aug. DC., a Malian medicinal plant, isolation and partial characterization (2005) J Ethnopharmacol., 101, pp. 204-214; Inngjerdingen, K.T., Coulibaly, A., Diallo, D., Michaelsen, T.E., Paulsen, B.S., A complement fixing polysaccharide from Biophytum petersianum Klotzsch, a medicinal plant from Mali, West Africa (2006) Biomacromolecules, 7, pp. 48-53; Inngjerdingen, M., Inngjerdingen, K.T., Patel, T.R., Allen, S., Chen, X., Rolstad, B., Morris, G.A., Diallo, D., Pectic poly-saccharides from Biophytum petersianum Klotzsch, and their activation of macrophages and dendritic cells (2008) Glycobiology, 18, pp. 1074-1084; Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275; McConville, M.J., Homans, S.W., Thomas-Oates, J.E., Dell, A., Bacic, A., Structures of the glycoinositolphospholipids from Leishmania major. A family of novel galactofuranose-containing glycolipids (1990) J Biol Chem, 265, pp. 7385-7394; Mellinger, C.G., Cipriani, T.R., Noleto, G.R., Carbonero, E.R., Mbm, O., Gorin, P.A.J., Iacomini, M., Chemical and immunological modifications of an arabinogalactan present in tea preparations of Phyllanthus niruri after treatment with gastric fluid (2008) Int J Biol Macromol, 43, pp. 115-120; Michaelsen, T.E., Gilje, A., Samuelsen, A.B., Hogasen, K., Paulsen, B.S., Interaction between human complement and a pectin type polysaccharide fraction, PMII, from the leaves of Plantago major L (2000) Scand J Immunol, 52, pp. 483-490; Nergard, C.S., Matsumoto, T., Inngjerdingen, M., Inngjerdingen, K., Hokputsa, S., Harding, S.E., Michaelsen, T.E., Paulsen, B.S., Structural and immunological studies of a pectin and a pectic arabi-nogalactan from Vernonia kotschyana Sch. Bip. ex Walp. (Asteraceae) (2005) Carbohydr Res, 340, pp. 115-130; Paulsen, B.S., Barsett, H., Bioactive pectic polysaccharides (2005) Adv Polym Sci, 186, pp. 69-101; Peterson, G.L., Review of the Folin phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall (1979) Anal Biochem, 100, pp. 201-220; Samuelsen, A.B., Paulsen, B.S., Wold, J.K., Otsuka, H., Kiyohara, H., Yamada, H., Knutsen, S.H., Characterization of a biologically active pectin from Plantago major L (1996) Carbohydr Polym, 30, pp. 37-44; Schmidgall, J., Schnetz, E., Hensel, A., Evidence for bioadhesive effects of polysaccharides and polysaccharide-containing herbs in an ex vivo bioad-hesion assay on buccal membranes (2000) Planta Med, 66, pp. 48-53; Shihata, I.M., El-Gendi, A.Y.I., Abd El-Malik, M.M., Pharmacochemical studies on saponin fraction of Opilia celtidifolia (1977) Planta Med, 31, pp. 60-67; Sims, I.M., Bacic, A., Extracellular polysaccharides from suspension cultures of Nicotiana plumbaginifolia (1995) Phytochemistry, 38, pp. 1397-1405; Stimpel, M., Proksch, A., Wagner, H., Lohmann-Matthes, M.L., Macrophage activation and induction of macrophage cytotoxicity by purified polysaccha-ride fractions from the plant Echinacea purpurea (1984) Infect Immun, 46, pp. 845-849; Sweet, M.J., Hume, D.A., Endotoxin signal transduction in macrophages (1996) J Leukoc Biol, 60, pp. 8-26; Togola, A., Diallo, D., Dembele, S., Barsett, H., Paulsen, B.S., Ethnopharmaco-logical survey of different uses of seven medicinal plants from Mali, (West Africa) in the regions Doila, Kolokani and Siby (2005) J Ethnobiol Ethnomed, 1, p. 7; Togola, A., Inngjerdingen, M., Diallo, D., Barsett, H., Rolstad, B., Michaelsen, T.E., Paulsen, B.S., Polysaccharides with complement fixing and macrophage stimulation activity from Opilia celtidifolia, isolation and partial characterisation (2007) J Ethnopharmacol, 115, pp. 423-431; Van Holst, G.J., Clarke, A.E., Quantification of arabinogalactan-protein in plant extracts by single radial gel diffusion (1985) Anal Biochem, 148, pp. 446-450; Wagner, H., Kraus, S., Jurcic, K., Search for potent immunostimulating agents from plants and other natural sources (1999) Immu-nomodulatory Agents from Plants, pp. 1-40. , Wagner H, editor Basel: BirkhÃ¤user; Yamada, H., Kiyohara, H., Complement-activating polysaccharides from medicinal herbs (1999) Immunomodulatory Agents from Plants, pp. 161-202. , Wagner H, editor. Basel: BirkhÃ¤user Verlag; Yamada, H., Kiyohara, H., Immunomodulating activity of plant polysaccha-ride structures (2007) Comprehensive Glycoscience-From Chemistry to Systems Biology, pp. 663-694. , Kamerling JP, editor Oxford: Elsevier
Depositing User:	Gordon Morris
Date Deposited:	19 May 2011 11:57
Last Modified:	01 Jul 2011 14:52
URI:	http://eprints.hud.ac.uk/id/eprint/10464

Item control for Repository Staff only:

View Item